Dental pulp stem cell transplantation facilitates neuronal neuroprotection following cerebral ischemic stroke - 18/06/22
, Mingchang Li a, ⁎ Abstract |
Objectives |
This study aimed to identify and evaluate the intracranial transplantation of dental pulp stem cells (DPSCs) as a possible ischemic stroke therapy that mitigates neuronal death/apoptosis.
Materials and methods |
DPSCs were isolated from the impacted third molars of healthy volunteers and then intracranially injected at 24 h post-ischemic stroke to Sprague Dawley rats that had been subjected to 2 h of middle cerebral artery occlusion. Neurological functional deficits were assessed using the modified neurological severity score (mNSS), and cerebral edema was quantified using brain water content. Neuronal death/apoptosis was indicated by TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, NeuN immunofluorescence and immunohistochemistry, and Western blot analysis of the protein expression of anti-apoptotic indicator of Bcl-2 and apoptotic indicators of Bax and caspase 3.
Results |
DPSC transplantation could ameliorate neurological dysfunction and brain edema, reduce infarct volume, decrease the percentage of TUNEL-positive nuclei, increase the number and percentage of NeuN-positive cells in ischemic penumbra, increase the ratio of Bcl-2 and Bax and down-regulate the production of caspase 3 in the cortical infarct zone.
Conclusions |
DPSC therapy via intracranial injection exerted remarkably neuroprotection mainly by inhibiting neuronal death/apoptosis.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Dental pulp stem cell (DPSC) implantation by intracranial injection was effective for ischemic stroke treatment. |
• | Transplantation of 1 × 105 DPSCs was feasible and functional for ischemic stroke treatment. |
• | DPSC implantation at 24 h after ischemic stroke was effective for ischemic stroke treatment. |
• | DPSC implantation attenuated ischemic injury through inhibiting neuronal apoptosis and reducing brain edema. |
Abbreviations : DPSCs, MSCs, BM-MSCs, AD-MSCs, NGF, BDNF, VEGF, tMCAO, ICA, TTC, TUNEL, mNSS, BBB, BMVECs, TJs
Keywords : Dental pulp stem cells, Transplantation, Ischemic stroke, Neuroprotection, Neuronal survival
Plan
Vol 152
Article 113234- août 2022 Retour au numéro
Article précédent
- Stem cells from human exfoliated deciduous teeth relieves Alzheimer’s disease symptoms in SAMP8 mice by up-regulating the PPARγ pathway
- Xiaoshuang Zhang, Tong Lei, Donghui Wang, Shanglin Cai, Zhongci Hang, Yanjie Yang, Wangyu Bi, Zhuangzhuang Xiao, Hongwu Du
- FTY720 decreases ceramides levels in the brain and prevents memory impairments in a mouse model of familial Alzheimer’s disease expressing APOE4
- Simone M. Crivelli, Qian Luo, Daan van Kruining, Caterina Giovagnoni, Marina Mané-Damas, Sandra den Hoedt, Dusan Berkes, Helga E. De Vries, Monique T. Mulder, Jochen Walter, Etienne Waelkens, Rita Derua, Johannes V. Swinnen, Jonas Dehairs, Erwin P.M. Wijnands, Erhard Bieberich, Mario Losen, Pilar Martinez-Martinez
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?