MicroRNAs miR-629-3p, miR-1202 and miR-1225-5p as potential diagnostic and surgery outcome biomarkers for mesial temporal lobe epilepsy with hippocampal sclerosis - 02/07/22

Doi : 10.1016/j.neuchi.2022.06.002

D. Gattás ^a, F.S.L. Neto ^a, P. Freitas-Lima ^a,^b, R. Bonfim-Silva ^a, S. Malaquias de Almeida ^a, M.L. de Assis Cirino ^a, D. Guimarães Tiezzi ^c, L.F. Tirapelli ^a, T.R. Velasco ^d, A.C. Sakamoto ^d, C.M. Matias ^d, C.G. Carlotti ^a, D.P.C. Tirapelli ^a,⁎
^a Department of Surgery and Anatomy, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto-SP, Brazil
^b Barão de Maua University Center, Ribeirao Preto-SP, Brazil
^c Department of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto-SP, Brazil
^d Department of Neurosciences and Behavioral Sciences, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto-SP, Brazil

^⁎Corresponding author. Ribeirão Preto Medical School, University of Sao Paulo, Av Bandeirantes, 3900, 14049-900 Ribeirao Preto-SP, Brazil.Ribeirão Preto Medical School, University of Sao PauloAv Bandeirantes, 3900Ribeirao Preto-SP14049-900Brazil

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Highlights

•	miR-629-3p, miR-1202 and miR-1225-5p are involved in GABAergic pathways.
•	miR-629-3p, miR-1202 and miR-1225-5p are hyper-expressed in epilepsy patients’ blood.
•	miR-629-3p, miR-1202 and miR-1225-5p are potential diagnostic biomarkers of MTLE-HS.
•	miR-1202 may be a potential surgery prognose biomarker in amygdala of MTLE-HS cases.

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Abstract

Background

Mesial temporal lobe epilepsy (MTLE) is a symptomatic epilepsy syndrome clinically characterized by high prevalence, pharmacoresistance, good surgical prognosis and hippocampal sclerosis (HS); however, no singular criteria can be considered sufficient for the MTLE-HS diagnosis. MicroRNAs (miRNAs) are small non-coding molecules that act as important gene-expression regulators at post-transcriptional level. Evidences on the involvement of miRNAs in epilepsy pathogenesis as well as their potential to be employed as biomarkers claim for investigations on miRNAs’ applicability as epilepsy diagnosis and prognosis biomarkers. Consequently, the present study aimed to evaluate the applicability of three specific miRNAs as biomarkers of diagnosis and surgical outcomes in adult patients with MTLE-HS.

Method

Hippocampus, amygdala and blood samples from 20 patients with MTLE-HS were analyzed, 10 with favorable surgical prognosis (Engel I) and 10 with unfavorable surgical prognosis (Engel III-IV). For the control groups, hippocampus and amygdala from necropsy and blood samples from healthy individuals were adopted. The miRNAs expression analysis was performed using Real-Time Quantitative Polymerase Chain Reaction for miRNAs highlighted from microarray as being involved in GABAergic neurotransmission.

Results

The miRNAs miR-629-3p, miR-1202 and miR-1225-5p were found to be hyper-expressed in MTLE-HS patients’ blood.

Conclusions

Our data suggest the existence of three circulating miRNAs (miR-629-3p, miR-1202 and miR-1225-5p) that could possibly act as additional tools in the set of factors that contribute to MTLE-HS diagnose.

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Keywords : Mesial temporal lobe epilepsy, MicroRNA, qRT-PCR, Amygdala, Hippocampus