Molecular determination, serotyping, antibiotic profile and virulence factors of group B Streptococcus isolated from invasive patients at Arabcare Hospital Laboratory, Palestine - 28/07/22
, Mahmoud Srour, PhD b, Shadi Hasan, MS b, Samir Khatib, PhD aHighlights |
• | Streptococcus agalactiae research studies are lacking in Palestine. |
• | We investigate the serotypes, virulent genes, and antibiotic profile of the invasive group B Streptococcus strains. |
• | Serotype III most is predominant among local isolates. |
• | Resistant group B Streptococcus strains are common in Palestine. |
• | Evidence-based infection prevention and antibiotic stewardship efforts are needed. |
Abstract |
Background |
Streptococcus agalactiae (group B Streptococcus) is beta-hemolytic, catalase negative, gram-positive cocci, recognized as main bacterial pathogen causing infections in newborns, infants, adults, and elderly people around the world. The aim of this study is to investigate group B Streptococcus samples recovered from invasive patients and determine serotype, virulent genes, and antibiotic-resistant profile of Streptococcus agalactiae in Palestine.
Methods |
A total of 95 group B Streptococcus strains were isolated from neonates, infants, pregnant and non-pregnant women and males at Arabcare Hospital Laboratory, Palestine, between the period of June 2018 and September 2020. Species identification was carried out through cultivation and conventional biochemical tests. A conventional Polymerase Chain Reaction (cPCR) was used to determine the 5 serotypes and virulent genes of the Streptococcus agalactiae strains. The antibiotic resistance test of group B Streptococcus was evaluated using Kirby-Bauer disk susceptibility. Sequencing and BLAST analysis were used to determine the relationship of the isolates in this study to worldwide isolates.
Results |
Serotype III (35%) was the major group B Streptococcus strains serotype causing invasive infections in neonates, infants, pregnant and nonpregnant women, and males, followed by serotypes V (19%), Ia, and II (15%), Ib (6%), respectively. All our isolates encoding for surface protein virulent factors, including a highly virulent gene (HvgA) were mostly found in strains isolated from pregnant women (12%). These group B Streptococcus strains exhibited a high rate of resistance to clindamycin (26%). The overall percentage of levofloxacin resistance was 11%, while vancomycin and ampicillin showed higher resistance, at 14.7 and 16% respectively. In addition, the phylogenetic relationship dendrogram illustrates that Streptococcus agalactiae isolated from an invasive patient (newborn) in Palestine was similar to strains found in China and Japan.
Conclusions |
The outcomes of this study demonstrate that resistant group B Streptococcus strains are common in Palestine, therefore, evidence-based infection prevention and antibiotic stewardship efforts are necessary.
Le texte complet de cet article est disponible en PDF.Key words : Neonates, Infant, Streptococcus agalactiae, Group B Streptococcus (GBS), Serotype, Antibiotic Resistance, Sequencing
Plan
| Conflict of interest: We declared no conflict of interest. |
|
| Ethics approval and consent to participate: Sample collection and analysis of the GBS isolates for this study were approved by Arabcare Hospital Laboratory ethical committee. |
Vol 50 - N° 8
P. 934-940 - août 2022 Retour au numéro
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