A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process - 31/08/22
, Ching-Hua Hsieh c, ⁎ Abstract |
Background |
Platelet-rich plasma (PRP) is beneficial for clinical applications in various medical fields. Although commercial PRP preparation kits are already available in the market, most of these kits employ centrifugation.
Methods |
We used a new cationic copolymer coating on a polyurethane (PU) sponge to promote platelet separation from the blood. This copolymer showed no cytotoxicity against cell viability or hemolysis. We further evaluated the efficiency of the new PRP preparation device by comparing it with that of a commercially available kit (RegenKit-THT).
Results |
We demonstrated that PRP obtained using copolymer device contains high concentrations of platelets and angiogenic growth factors (epidermal growth factor, vascular endothelial growth factor-A, growth differentiation factor 2, and interleukin-8). The separated PRP also displayed beneficial effects on cell migration, angiogenesis, and matrix metalloproteinase gene expression.
Conclusion |
Based on these results, we developed a cationic copolymer-coated PU sponge as a PRP preparation device without the need for any centrifugation.
Le texte complet de cet article est disponible en PDF.Highlights |
• | We described the positive ionic copolymers (PICs) with platelet adsorption ability. |
• | PICs adsorb platelet without destroying RBC and with a good WBC removal. |
• | PICs provide a convenient method for PRP separation without centrifugation. |
Abbreviations : ATR-FTIR, CBC, EDS, EGF, FGF, IGF, IL, L-PRF, L-PRP, MIPA, MMP, NMR, PDMAEMA, PIC, PRP, P-PRF, P-PRP, PU, SEM, VEGF, WBCs
Keywords : Platelet-rich plasma, Copolymer, Centrifugation, Angiogenesis, Growth factors
Plan
Vol 153
Article 113481- septembre 2022 Retour au numéro
Article précédent
- Upregulation of PARG in prostate cancer cells suppresses their malignant behavior and downregulates tumor-promoting genes
- Yaroslava Karpova, Sarah J. Johnson, Guillaume Bordet, Danping Guo, Atreyi Ghatak, Dmitriy A. Markov, Alexei V. Tulin
- The nature inspired peptide [T20K]-kalata B1 induces anti-tumor effects in anaplastic large cell lymphoma
- Judith Lind, Roland Hellinger, Petra Kudweis, Herwig P. Moll, Jasmin Gattringer, Kathrin Thell, Sophie Edtmayer, Christian W. Gruber, Dagmar Stoiber, Karoline Kollmann
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?