Shared signatures and divergence in skin microbiomes of children with atopic dermatitis and their caregivers - 04/10/22
, Niranjan Nagarajan, PhD a, e, ⁎ , Elizabeth Huiwen Tham, MRCPCH e, f, ⁎ Abstract |
Background |
Atopic dermatitis (AD) is a common chronic skin condition in children (15-20%) that can significantly impair their quality of life. As a result of its relapsing nature and enrichment of Staphylococcus aureus during flares, clinical management can include eradicating S aureus from the skin of children; however, this does not extend to their healthy caregivers, who are potential reservoirs.
Objective |
Our aim was to understand skin microbiome sharing and microbial features in children with AD and their healthy adult caregivers.
Methods |
We utilized whole-metagenome profiling at 4 body sites (volar forearm, antecubital fossae, cheeks, and lesions) in combination with sequencing of S aureus isolates to characterize a cohort of children with AD and their healthy caregivers (n = 30 families) compared to matched pairs from control households (n = 30 families).
Results |
Metagenomic analysis revealed distinct microbiome configurations in the nonlesional skin of AD children and their healthy caregivers versus controls, which were sufficient to accurately predict case–control status (area under the receiver operating characteristic curve > 0.8). These differences were accompanied by significant microbiome similarity between children and their caregivers, indicating that microbiome sharing may play a role in recurrent disease flares. Whole-genome comparisons with high-quality S aureus isolate genomes (n = 55) confirmed significant strain sharing between AD children and their caregivers and AD-specific enrichment of strains expressing enterotoxins Q and K/K2.
Conclusion |
Our results highlight the distinctive skin microbiome features of healthy caregivers for children with AD and support their inclusion in strategies for the treatment of recurrent pediatric AD.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : Atopic dermatitis, caregivers, household contact, skin microbiome, Staphylococcus aureus
Abbreviations used : A/H ratio, Ac, AD, ANI, AUC, Ch, CSS, MRSA, MSSA, SCORAD, SEQ, Vf
Plan
| This research was supported by grant funding from the National University Health System Leadership in Academic Medicine program (PFFR September 2016); Master of Clinical Investigation, Yong Loo Lin School of Medicine, National University of Singapore, and NUS Biomedical Institute for Global Health Research and Technology (BIGHEART) Joint Research Grant 2018, administered by the Institute for Health Innovation and Technology, National University of Singapore, and an A∗STAR BMRC EDB IAF-PP grant (H18/01/a0/016) entitled “Asian Skin Microbiome Program.” M.C. and A.S.L.T. are research fellows receiving core funding from the A∗STAR Graduate Academy. E.H.T. is supported by a National Medical Research Council (NMRC) research training fellowship (MH 095:003008-225) and an NMRC transition award (MOH-000269). Computational work was partially performed using the resources of the National Supercomputing Centre, Singapore (www.nscc.sg). |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 150 - N° 4
P. 894-908 - octobre 2022 Retour au numéro
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