Lipid mediators are detectable in the nasal epithelium and differ by asthma status in female subjects - 04/10/22
, Jonathan Manke, BA b, Monica Campbell, MS a, Michael Armstrong, BS b, Meher Preethi Boorgula, MS a, Gabriela Pinheiro, MS c, Cinthia Vila Nova Santana, PhD c, Rasika A. Mathias, PhD d, Kathleen C. Barnes, PhD a, Alvaro Cruz, MD, PhD c, Nichole Reisdorph, PhD b, Camila A. Figueiredo, PhD c, eAbstract |
Background |
Lipid mediators, bioactive products of polyunsaturated fatty acid metabolism, contribute to inflammation initiation and resolution in allergic diseases; however, their presence in lung-related biosamples has not been fully described.
Objective |
We aimed to quantify lipid mediators in the nasal airway epithelium and characterize preliminary associations with asthma.
Methods |
Using liquid chromatography–mass spectrometry, we conducted a pilot study to quantify 56 lipid mediators from nasal epithelial samples collected from 11 female participants of an outpatient asthma clinic and community controls (aged 30-55 years). We examined the presence of each compound using descriptive statistics to test whether lipid mediators could distinguish subjects with asthma (n = 8) from control subjects (n = 3) using linear regression and partial least squares discriminant analysis.
Results |
Fifteen lipid mediators were detectable in all samples, including resolvin (Rv) D5 (RvD5), with the highest median concentrations (in pg/μg protein) of 13-HODE (126.481), 15-HETE (32.869), and 13-OxoODE (13.251). From linear regression adjusted for age, prostaglandin E2 (PGE2) had a trend (P < .1) for higher concentrations in patients with severe asthma compared to controls (mean difference, 0.95; 95% confidence interval, −0.04 to 1.95). Asthma patients had higher scores on principal component 3 compared to controls (mean difference, 2.42; 95% confidence interval, 0.89 to 3.96), which represented lower levels of proresolving 15-HEPE, 19,20-DiHDPA, RvD5, 14-HDHA, 17-HDHA, and 13-HOTrE. Most of these compounds were best at discriminating asthma cases from controls in partial least squares discriminant analysis.
Conclusion |
Lipid mediators are detectable in the nasal epithelium, and their levels distinguish asthma cases from controls.
Le texte complet de cet article est disponible en PDF.Key words : Lipids, asthma, nasal airway epithelium, proresolving lipids, oxylipins, resolvins, PUFAs, EPA, DHA, AA
Abbreviations used : AA, ALA, DGLA, DHA, DPA, EPA, LA, LT, LX, MMA, PC, PGE2, RV
Plan
| This work was supported by National Institutes of Health (NIH)–National Heart, Lung, and Blood Institute (NHLBI) grant R01HL104608 to K.C.B. and by NIH–National Center for Research Resources 1S10OD010366-01A1 and NIH-NHLBI grant R01HL123385 to N.R. This study was financed in part by Programa de Apoio a Núcleos Emergentes (PRONEM)–Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) (Edital: 009/2014; Pedido: 8305/2014; Termo de outorga: PNE0003/2014). |
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| Disclosure of potential conflict of interest: K. C. Barnes receives royalties from UpToDate and is employed by Tempus. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 150 - N° 4
P. 965 - octobre 2022 Retour au numéro
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