Diagnostics for particular populations outside of traditional health care settings have driven development of point-of-care testing (POCT). POCT is particularly suitable for patients with infections conditions to mitigate infection spread via its provision in venues with less concern for stigma. Patients in rural or resource-limited settings can benefit from POCT through more timely diagnosis and linkage-to-care. However, gaps in POCT availability compared with better-resourced, urban counterparts persist. Leveraging communication technologies, using mobile clinics, changing national health care policy, and implementing novel geospatial science concepts can address limitations of POCT use and reduce POCT access gaps in these settings.