Antagonizing interleukine-8 improves the durability of biological aortic valves: Translation of fundamental results using a model of subcutaneous aortic valve implantation in rats into clinical application such as cardiac surgery - 12/05/23
Résumé |
Introduction |
Aortic valvle stenosis is a common disease, only surgical therapy is recommended.
Objective |
Using the model of subcutaneous aortic valve implantation in rats described first time by Fishbein et al. in 1982, we test the hypothesis that antagonizing Interleukine-8 (IL-8) receptors reduces calcification of the pig aortic valves.
Method |
Pigs Hearts were explanted directly from the slaughter house. Aortic valvular leaflets were then implanted subcutaneously within 4hours on the back of Sprague Dawley young rats. Every day for a total period of 14days, rats received either local subcutaneous injection either intra-peritoneal injection of drug, either none drug. The drug was SCH527123, which is an antagonist of CXCR-1 and CXCR-2, receptors of IL-8. The 3 groups were sacrificed at the end of 14 days after a blood sampling and the aortic valvular leaflets were explanted to be analyzed. The analysis consisted of immuno-histochemistry coloration, calcium dosage and western-blot analysis.
Results |
GA increases calcification. Inflammation is present highly concomitant with calcification. Macroscopical findings confirm the part of red blood cells in the mechanism of calcification.
Conclusion |
Parallel with clinical results can be made as the age of patient.
The use of antagonist of CXCR-1 or CXCR-2 in the treatment of pre-incubation of bioprosthesis or oral prescriptions concomitant of the aortic valve replacement may be a lead for improving the durability of aortic bioprosthesis.
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Vol 15 - N° 2
P. 223 - mai 2023 Retour au numéro
Article précédent
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