Quercus coccinea Münchh leaves polyphenols: Appraisal acute lung injury induced by lipopolysaccharide in mice - 27/05/23

Doi : 10.1016/j.biopha.2023.114765

Elsayed K. El-Sayed ^a,^{^{1
These authors share the first authorship.}}, Reham R. Ibrahim ^b,^{^{1
These authors share the first authorship.}}, Asmaa A. Ahmed ^a, Mohamed A. Khattab ^c, Lo-Yun Chen ^d, Kuei-Hung Lai ^d,^e,^f,^⁎ , Fatheya S. El Shaarawy ^b,^{^{2
These authors share the last authorship.}}, Nashwa F. Tawfik ^b,^{^{2
These authors share the last authorship.}}, Fatma A. Moharram ^b,^{^{2
These authors share the last authorship.}}
^a Pharmacology and Toxicology Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo 11795, Egypt
^b Pharmacognosy Department, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt
^c Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
^d Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan
^e PhD Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan
^f Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan

^⁎Corresponding author at: Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan.Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical UniversityTaipei11031Taiwan

Sous presse. Épreuves corrigées par l'auteur. Disponible en ligne depuis le Saturday 27 May 2023
Cet article a été publié dans un numéro de la revue, cliquez ici pour y accéder

Abstract

Genus Quercus is a well-known source for its polyphenolic content and important biological activity. Plants belonging to the Quercus genus were traditionally used in asthma, inflammatory diseases, wound healing, acute diarrhea, and hemorrhoid. Our work intended to study the polyphenolic profile of the Q. coccinea (QC) leaves and to assess the protective activity of its 80% aqueous methanol extract (AME) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Together, the potential molecular mechanism was investigated. Nineteen polyphenolic compounds (1–18), including tannins, flavone, and flavonol glycosides. Phenolic acids and aglycones were purified and identified from the AME of QC leaves. Treatment with AME of QC showed an anti-inflammatory effect evidenced by a remarkable decline in the count of white blood cells and neutrophils which was in harmony with decreasing the levels of high mobility group box-1, nuclear factor kappa B, tumor necrosis factor-α, and interleukin 1 beta. In addition, the antioxidant activity of QC was documented through the significant reduction in malondialdehyde level and elevation of reduced glutathione level and superoxide dismutase activity. Furthermore, the mechanism involved in the pulmonary protective effect of QC involved the downregulation of the TLR4/MyD88 pathway. The AME of QC showed a protective effect against LPS-induced ALI through the powerful anti-inflammatory and antioxidant activities which are linked to its abundancy with polyphenols.

Le texte complet de cet article est disponible en PDF.

Graphical Abstract

ga1

Le texte complet de cet article est disponible en PDF.

Highlights

●	Quercus coccinea was used traditionally in asthma, inflammation diseases.
●	Polyphenols are characterized by many biological activities.
●	Lung injury was induced by lipopolysaccharide in mice.
●	QC attenuates lung inflammation by modulating the MyD88-dependent TLR4 signaling.
●	QC exhibited antioxidant activity via modulating oxidative stress/NF-κB signaling.