Polyphenol-enriched Desmodium elegans DC. ameliorate scopolamine-induced amnesia in animal model of Alzheimer’s disease: In Vitro, In Vivo and In Silico approaches - 13/08/23
, Hammad Ullah d, Alam Zeb e, Mehreen Ghufran f, Shah Fahad g, Muhammad Ayaz b, ⁎ , Maria Daglia d, h, ⁎ Abstract |
The current study aims to quantify HPLC-DAD polyphenolics in the crude extracts of Desmodium elegans, evaluating its cholinesterase inhibitory, antioxidant, molecular docking and protective effects against scopolamine-induced amnesia in mice. A total of 16 compounds were identified which include gallic acid (239 mg g -1 ), p -hydroxybenzoic acid (11.2 mg g -1 ), coumaric acid (10.0 mg g -1 ), chlorogenic acid (10.88 mg g -1 ), caffeic acid (13.9 mg g -1 ), p -coumaroylhexose (41.2 mg g -1 ), 3- O -caffeoylquinic acid (22.4 mg g -1 ), 4- O -caffeoylquinic acid (6.16 mg g -1 ), (+)-catechin (71.34 mg g -1 ), (-)-catechin (211.79 mg g -1 ), quercetin-3- O -glucuronide (17.9 mg g -1 ), kaempferol-7- O -glucuronide (13.2 mg g -1 ), kaempferol-7- O -rutinoside (53.67 mg g -1 ), quercetin-3-rutinoside (12.4 mg g -1 ), isorhamnetin-7- O -glucuronide (17.6 mg g -1 ) and isorhamnetin-3- O -rutinoside (15.0 mg g -1 ). In a DPPH free radical scavenging assay, the chloroform fraction showed the highest antioxidant activity, with an IC 50 value of 31.43 µg mL -1 . In an AChE inhibitory assay, the methanolic and chloroform fractions showed high inhibitory activities causing 89% and 86.5% inhibitions with IC 50 values of 62.34 and 47.32 µg mL -1 respectively. In a BChE inhibition assay, the chloroform fraction exhibited 84.36% inhibition with IC 50 values of 45.98 µg mL -1 . Furthermore, molecular docking studies revealed that quercetin-3-rutinoside and quercetin-3- O -glucuronide fit perfectly in the active sites of AChE and BChE respectively. Overall, the polyphenols identified exhibited good efficacy, which is likely as a result of the compounds' electron-donating hydroxyl groups (-OH) and electron cloud density. The administration of methanolic extract improved cognitive performance and demonstrated anxiolytic behavior among tested animals.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Genus Desmodium including Desmodium elegans DC. have ethnomedicinal uses in the management of neurological disorders. |
• | HPLC-DAD polyphenolics quantification revealed significant amount of sixteen polyphenolic compounds. |
• | Considerable in vitro antioxidant, cholinesterase inhibitory potentials were observed for the samples. |
• | Molecular docking studies revealed that the active compounds fit well in the target site of the enzymes. |
• | Our tested samples improve special memory and cognitive performance in animal models. |
Abbreviations : Aβ, Ach, AChE, AD, BChE, BHA, BHT, CNS, De.Chf, De.Cme, De.EtAc, De.Hex, FDA, HPLC-DAD, NFTs, SWM, TL
Keywords : Desmodium elegans DC. , Alzheimer’s disease, Oxidative stress, Amnesia, Polyphenolics, Molecular docking
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Vol 165
Article 115144- septembre 2023 Retour au numéro
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