Next-generation analytical platforms for antioxidant capacity assessment: The urge for realistic and physiologically relevant methods - 13/08/23
Abstract |
Bioactive compounds, such as carotenoids, alkaloids, and phenolics, are well known because of their alleged health benefits when consumed regularly in a balanced healthy diet. Some well-documented bioactivities are antioxidant, antihypertensive, antihyperglycemic, antilipidemic, anti-obesity, anti-inflammatory, and antimicrobial capacities. Trying to associate the chemical composition of distinct sources and their bioactivity using in vitro methods, several assays have been developed, implemented, and optimised to recapitulate human physiological conditions. However, in most cases, pitfalls are apparent, and no single test tube-based assay can predict in vivo responses. The need for a more physiologically relevant cell-based method to evaluate the antioxidant capacity of putative antioxidants is apparent. Therefore, in this Review, the current state-of-the-art in food science and nutrition is aligned with cell biology/bioengineering approaches to propose combining in vitro digestion and absorption to obtain a bioavailable fraction containing antioxidants. Overall, human plasma, 2-dimensional human cell lines, such as erythrocytes, lymphocytes, hepatocytes, enterocytes and, ultimately, 3-dimensional spheroids (organoids) could be used as biologically relevant models to assess the antioxidant activity of compounds, foods, and nutraceuticals. This versatile approach is deemed suitable, accurate, reproducible, and physiologically relevant to evaluate the protective effects of antioxidants against ROS-mediated oxidation in vitro.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Chemical antioxidant activity does not reflect cellular physiological conditions. |
• | Bioavailability and biotransformation of antioxidants are currently ignored. |
• | In vitro digestion and absorption using Caco-2 co-culture should be used. |
• | Cellular antioxidant activity should be performed using bioavailable fractions. |
• | 2D/3D human cell models must be used in antioxidant activity tests. |
Abbreviations : ABTS, AAPH, DPPH, 2D, 3D, RO , , BiF, BaF, CVDs, CAT, CAA, hs-CRP, CUPRAC, ERYCA, FRAP, FOPs, FCRC, GR, GPx, HO-1, HDL, H 2 O 2, , HOO , , HORAC, OH, IL2, LDL, MDA, NDs, NO 2 , , NOD-, Nrf2, ORAC, HO 2 , , ROO , , ONOO , , ROS, RT-PCR, SOD, O 2 - , tBHP, TBARS, TOSC, TRAP, TRC, WB
Keywords : Reactive oxygen species, Analytical methods, Bioactive compounds, Natural products, Cell culture, Organoids
Plan
Vol 165
Article 115155- septembre 2023 Retour au numéro
Article précédent
- Dexmedetomidine abates myocardial ischemia reperfusion injury through inhibition of pyroptosis via regulation of miR-665/MEF2D/Nrf2 axis
- Lingyan Wang, Jin Liu, Zhuoran Wang, Xi Qian, Yu Zhao, Quan Wang, Na Dai, Yuhan Xie, Weijun Zeng, Wei Yang, Xiangfeng Bai, Yuqiao Yang, Jinqiao Qian
- In-vivo anti-diabetic and anti-hyperlipidemic effects of natural metabolites from resin of Commiphora mukul and their in-silico to in-vitro target fishing
- Waseem Ul Islam, Faizullah Khan, Muhammad Waqas, Saeed Ullah, Sobia Ahsan Halim, Najeeb Ur Rehman, Hanif Khan, Mohamed H. Mahmoud, Gaber El-Saber Batiha, Ajmal Khan, Ahmed Al-Harrasi
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?