A simplified and efficient method for single-step, targeted gene deletion in mycobacteria - 10/06/24
Doi : 10.1016/j.tube.2024.102520
Yahav Bracha, Daniel Barkan ⁎ 
Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
⁎Corresponding author.
Abstract |
Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.
Le texte complet de cet article est disponible en PDF.Keywords : Mycobacteria, Genetic manipulation, Gene deletion
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© 2024
Elsevier Ltd. Tous droits réservés.
Vol 147
Article 102520- juillet 2024 Retour au numéro
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