Sulforaphane improves post-resuscitation myocardial dysfunction by inhibiting cardiomyocytes ferroptosis via the Nrf2/IRF1/GPX4 pathway - 20/09/24
, Mao Zhang a, b, c, ⁎ Abstract |
Background |
Ferroptosis is an important type of cell death contributing to myocardial dysfunction induced by whole body ischemia reperfusion following cardiac arrest (CA) and resuscitation. Sulforaphane (SFN), known as the activator of the nuclear factor E2-related factor 2 (Nrf2), has been proven to effectively alleviate regional myocardial ischemia reperfusion injury. The present study was designed to investigate whether SFN could improve post-resuscitation myocardial dysfunction by inhibiting cardiomyocytes ferroptosis and its potential regulatory mechanism.
Methods and results |
An in vivo pig model of CA and resuscitation was established. Hypoxia/reoxygenation (H/R)-stimulated AC16 cardiomyocytes was constructed as an in vitro model to simulate the process of CA and resuscitation. In vitro experiment, SFN reduced ferroptosis-related ferrous iron, lipid reactive oxygen species, and malondialdehyde, increased glutathione, and further promoted cell survival after H/R stimulation in AC16 cardiomyocytes. Mechanistically, the activation of Nrf2 with the SFN decreased interferon regulatory factor 1 (IRF1) expression, then reduced its binding to the promoter of glutathione peroxidase 4 (GPX4), and finally recovered the latter’s transcription after H/R stimulation in AC16 cardiomyocytes. In vivo experiment, SFN reversed abnormal expression of IRF1 and GPX4, inhibited cardiac ferroptosis, and improved myocardial dysfunction after CA and resuscitation in pigs.
Conclusions |
SFN could effectively improve myocardial dysfunction after CA and resuscitation, in which the mechanism was potentially related to the inhibition of cardiomyocytes ferroptosis through the regulation of Nrf2/IRF1/GPX4 pathway.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | SFN significantly improved myocardial dysfunction and alleviated cardiac injury after cardiac arrest and resuscitation. |
• | SFN activated Nrf2 to produce post-resuscitation myocardial protection through inhibition of cardiomyocytes ferroptosis. |
• | Nrf2 activation by SFN alleviated cardiomyocytes ferroptosis by recovering GPX4 transcriptional through inhibiting IRF1. |
Abbreviations : CA, SFN, Nrf2, IRF1, GPX4, CPR, I/R, ROS, H/R, LDH, Fe2+, MDA, GSH, ROSC, ChIP
Keywords : Cardiac arrest, Cardiopulmonary resuscitation, Myocardial dysfunction, Ferroptosis, Sulforaphane, Nuclear factor E2-related factor 2, Interferon regulatory factor 1, Glutathione peroxidase 4
Plan
Vol 179
Article 117408- octobre 2024 Retour au numéro
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