In this review article, we describe the development and application of diffusion-based MR imaging methods for studying glymphatic physiology. Fluid exchange and solute transport are the 2 key components of the glymphatic system. Here we describe the use of low b-value imaging, free water fraction imaging, and diffusion time sensitization to leverage cerebral spinal fluid, as well as interstitial fluid motion in the parenchyma. We also describe multiple b-value diffusion imaging to better delineate diffusion components within the brain. Finally, we touch upon newer approaches that use advanced models of the diffusion signal, including high b-value imaging.