Bacterial biliary tract infections are increasingly recognized in dogs and should be considered in any dog presenting with vomiting, anorexia, and jaundice, particularly if they have abdominal discomfort or pyrexia. Commonly recognized clinicopathologic findings include increased liver enzyme activities, hyperbilirubinemia, and an inflammatory leucogram. Hepatobiliary ultrasound forms a key component of the diagnostic approach to these cases, aiding the identification of dogs that require urgent surgical management due to biliary tract rupture, or that have another surgical condition. Ultrasound also allows guided percutaneous bile sampling to gain a sample for cytology and culture, guiding rational antimicrobial therapy.