Superficial mycoses present several clinical manifestations, ranging from lesions of the skin and its appendages to progression into disseminated infections. Antifungal resistance to currently available drugs is a global public health concern, necessitating novel antimicrobial compounds. Metal nanoparticles or simvastatin (SIM), via drug repositioning, represent promising avenues. The aims of this study were to synthesize and characterize silver nanoparticles (AgNPs) and selenium nanoparticles (SeNPs) and to evaluate their antifungal activity in combination with simvastatin against T. mentagrophytes, T. rubrum, N. gypsea, C. albicans, C. tropicalis , and M. pachydermatis . Nanoparticles were biosynthesized using the supernatant of L. ornata and characterized by UV–Vis, FTIR, zeta potential analysis, DLS, and EDXRF. Their antifungal activity was assessed via broth microdilution (MIC) and checkerboard assays to evaluate agent interactions. MIC values ranged from 1 to 8.7 µg/mL for AgNPs, 194.5 to 778 µg/mL for SeNPs, and 78 to 312.5 µg/mL for simvastatin. A synergistic effect was observed between AgNPs and simvastatin against T. rubrum and T. mentagrophytes . The L. ornata strain demonstrated the ability to synthesize AgNPs and SeNPs, though further research is required to optimize SeNP production. The tested compounds displayed antifungal activity against most of the species analyzed, highlighting their potential as promising alternatives for treating superficial mycoses and combating antifungal resistance.