Caged-hypocrellin-mediated antimicrobial photodynamic therapy as a dual-action strategy for fungal clearance and immune response regulation in drug-resistant Candida auris wound infections - 18/12/25
, Wenjie Fang, MD f, ⁎ , Yuping Ran, MD b, ⁎ Abstract |
Background |
Multidrug-resistant Candida auris is designated by the World Health Organization as a critical priority pathogen. Its bloodstream infections have ∼40% mortality and predominant cutaneous origin, necessitating early local control. With limited treatment options, new antifungal strategies are urgently needed. Antimicrobial photodynamic therapy (aPDT) has shown promise for superficial infections, although its immune-modulating mechanisms remain unclear.
Objective |
To assess antifungal and immunomodulatory effects of aPDT on multidrug-resistant C. auris skin wound infection.
Methods |
A murine wound model using C. auris CBS14918 was treated with COP1T-HA-based aPDT. Fungal burden, wound healing, immune profiles, and transcriptomic responses were evaluated. COP1T-only and dark groups were excluded from immunological analyses because prior validation revealed a lack of efficacy.
Results |
aPDT significantly reduced fungal burden and accelerated wound healing. It enhanced local infiltration of myeloid cells, B cells, γδ T cells, and type 2 innate lymphoid cells, while controlling systemic inflammation. Transcriptomic data confirmed a balanced cytokine profile supporting fungal clearance and tissue repair.
Limitations |
The study used only animal models, as proof-of-concept therapies cannot be ethically tested in humans.
Conclusions |
aPDT eradicates drug-resistant C. auris and enhances wound healing via immune modulation. These effects support its potential as a preclinical strategy when human studies are ethically prohibited.
Le texte complet de cet article est disponible en PDF.Key words : Candida auris, immune response, multidrug-resistance, photodynamic therapy, wound healing
Abbreviations used : aPDT, IFN, IL, ILC2, MHC, NF-κB, NOD, PBMC, PBS, PDT, ROS, TGF, TNF
Plan
| Funding sources: This work was supported by the National Natural Science Foundation of China [ 82302546 , 82202543 ]; the Guangxi Science and Technology Program [ AB25069013 ]; the Project of the Ministry of Science and Technology [ 2022YFC2504803 ]; the National Key Research and Development Program of China [ 2022YFC2504800 ]; and the HX-Academician Project of West China Hospital, Sichuan University [ HXYS19003 ]. |
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| IRB approval status: All animal experimental protocols were licensed by the Animal Ethics Committee of West China Hospital of Sichuan University (20220803003) and complied with all relevant ethical regulations. |
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| Data availability: The authors declare that the data supporting the findings of this study are available within the paper and the supporting information or are available from the authors upon request. |
Vol 94 - N° 1
P. 73-80 - janvier 2026 Retour au numéro
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