Diagnostic performances of Anyplex MTB/NTM Real-time detection vs Xpert® MTB/RIF Ultra for tuberculosis among people living with human immunodeficiency virus with low CD4 count - 24/12/25
Highlights |
• | The respective performances of Anyplex and Xpert in PLHIV with low CD4 count were assessed. |
• | The two assays showed similar MTC diagnostic performances in respiratory samples. |
• | Anyplex showed higher specificity and lower sensitivity in non-respiratory samples. |
• | The differences in diagnostic performance were observed based on CD4 count. |
Abstract |
Objectives |
The aim of this study was to evaluate the performances of Anyplex® MTB/NTM Real-time Detection and Xpert MTB/RIF Ultra for tuberculosis (TB) disease in people living with Human Immunodeficiency Virus (PLHIV) with low CD4 + T lymphocyte cell (CD4) count.
Patients and methods |
A retrospective observational study included respiratory and non-respiratory specimens from PLHIV stratified based on their CD4 count (<200 vs. ≥ 200 cells/µL) using Anyplex® MTB/NTM Real-time Detection or Xpert® MTB/RIF Ultra.
Results |
In PLHIV with low CD4 count (<200 cells/µL), Anyplex demonstrated comparable sensitivity (81.4 % vs. 81.8 %) and specificity (78.7 % vs. 78.5 %) to Xpert for MTC detection in respiratory samples, but lower sensitivity (81.8 % vs. 100 %) and higher specificity (83.3 % vs. 66.6 %) than Xpert in non-respiratory samples.
Conclusions |
Whereas the two methodologies showed similar MTC diagnostic performance in respiratory samples from PLHIV with low CD4 levels, Anyplex showed higher specificity and lower sensitivity than Xpert in non-respiratory samples.
Le texte complet de cet article est disponible en PDF.Keywords : Tuberculosis, HIV, Real-time polymerase chain reaction, CD4
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Vol 56 - N° 1
Article 105191- janvier 2026 Retour au numéro
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