To evaluate the quality, popularity, and educational content of YouTube videos on intravesical botulinum toxin injection (IBI).

A YouTube search was conducted on 10 May 2025 using 4 keywords related to IBI. Eligible videos were categorized into 3 groups: academic institutions, health information websites, and physicians or private medical organizations. Their quality and educational value were then evaluated using the Global Quality Score (GQS), the Patient Education Materials Assessment Tool for Audiovisual Materials (PEMAT-A/V), and a 15-item IBI-Specific Checklist (IBI-SC). Group comparisons were conducted using Pearson’s chi-square test and the Kruskal-Wallis test, and Pearson correlation was employed to evaluate relationships among the assessment tools.

Comparison among groups based on the sources of the final set of 45 videos revealed that the physician or private medical organization group performed significantly worse than the other groups in the analysis of PEMAT-A/V Understandability (U), Actionability (A), and IBI-SC scores ( P   < .001). GQS categories also differed significantly among videos from different sources, with the academic institutions group performing the best ( P   < .001). In the correlation analysis, a strong correlation was observed between PEMAT-A/V U and PEMAT-A/V A ( r  = 0.71, P   < .001), while moderate correlations were found between PEMAT-A/V U and IBI-SC ( r  = 0.58, P   < .001) and between PEMAT-A/V A and IBI-SC ( r  = 0.42, P  = .004).

The effectiveness of educational content varied significantly by source, with videos from academic institutions demonstrating the highest quality. Our findings highlight the need for standardized, open-access health information resources tailored to specific educational purposes.