Cellular Image Analysis and Imaging by Flow Cytometry - 09/08/11
, William E. Ortyn, BS, Luchuan Liang, PhD, Vidya Venkatachalam, PhD, Philip Morrissey, PhD
Corresponding author. Amnis Corporation, 2505 Third Avenue, Suite 210, Seattle, WA 98121.Abstract |
Imaging flow cytometry combines the statistical power and fluorescence sensitivity of standard flow cytometry with the spatial resolution and quantitative morphology of digital microscopy. The technique is a good fit for clinical applications by providing a convenient means for imaging and analyzing cells directly in bodily fluids. Examples are provided of the discrimination of cancerous from normal mammary epithelial cells and the high-throughput quantitation of fluorescence in situ hybridization (FISH) probes in human peripheral blood mononuclear cells. The FISH application will be enhanced further by the integration of extended depth-of-field imaging technology with the current optical system.
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| The authors of this article are shareholders and in the employ of Amnis Corporation. This work was supported partially by grant numbers 9 R44 CA01798-02 and 1 R43 GM58956-01 from the National Institutes of Health and contract number NNA05CR09C from the National Aeronautics and Space Administration. |
Vol 27 - N° 3
P. 653-670 - septembre 2007 Retour au numéro
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