Automated Forensic DNA Purification Optimized for FTA Card Punches and Identifiler STR-based PCR Analysis - 09/08/11
, Michelle Thomas, MS b, Karl Reich, PhD b
Corresponding author.Abstract |
Forensic labs globally face the same problem—a growing need to process a greater number and wider variety of samples for DNA analysis. The same forensic lab can be tasked all at once with processing mixed casework samples from crime scenes, convicted offender samples for database entry, and tissue from tsunami victims for identification. Besides flexibility in the robotic system chosen for forensic automation, there is a need, for each sample type, to develop new methodology that is not only faster but also more reliable than past procedures. FTA is a chemical treatment of paper, unique to Whatman Bioscience, and is used for the stabilization and storage of biological samples. Here, the authors describe optimization of the Whatman FTA Purification Kit protocol for use with the AmpFlSTR Identifiler PCR Amplification Kit.
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| This article was originally published in JALA; August 2005. |
Vol 27 - N° 1
P. 183-191 - mars 2007 Retour au numéro
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