A Buoyancy-Driven Compact Thermocycler for Rapid PCR - 09/08/11
Doi : 10.1016/j.cll.2007.01.004
Nitin Agrawal, PhD a 
, Victor M. Ugaz, PhD b, ⁎
, Victor M. Ugaz, PhD b, ⁎ a Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital and Harvard Medical School, Boston, MA 02114, USA
b Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA
Corresponding author.Abstract |
The authors have designed a novel convective flow-based thermocycling system capable of performing high-speed DNA amplification via the polymerase chain reaction in a simplified and inexpensive format. Successful amplification of a 191 bp influenza-A target is demonstrated within 25 minutes using a 10 μL reaction volume with no modification to standard laboratory protocols. The system is simple to assemble and can be readily integrated with existing laboratory instrumentation for automated operation.
Le texte complet de cet article est disponible en PDF.Keywords : PCR, DNA, thermocycler, convective flow, thermosyphon
Plan
© 2007
Elsevier Inc. Tous droits réservés.
Vol 27 - N° 1
P. 215-223 - mars 2007 Retour au numéro
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