p63 Gene Expression Study and Early Bladder Carcinogenesis - 09/08/11
, Ivan Bièche c, Delphine Dargère a, Sophie Ferlicot d, Ingrid Laurendeau c, Gérard Benoît e, Annick Vieillefond f, Catherine Verret g, Michel Vidaud c, Fréderique Capron b, Pierre Bedossa a, Valérie Paradis a
Reprint requests: Eva Compérat, M.D., Laboratoire d’Anatomie et Cytologie Pathologique, Hôpital La Pitié Salpêtrière, 83, Boulevard de l’Hôpital, Paris 75013 France.Résumé |
Objectives |
Urothelial carcinoma is a frequent and aggressive cancer. To gain better insight into the early molecular mechanisms of bladder carcinogenesis, this study analyzed the expression levels of four selected genes (uroplakin II, TATA-BOX-binding protein (TBP/RNA) control gene (NM_00394), and the two main isoforms TATp63 and ΔNp63 of p63).
Methods |
We used real-time quantitative reverse transcriptase-polymerase chain reaction in dissected tissues from normal bladder, noninvasive cancer, and muscle-invasive bladder carcinoma (n = 49). The gene expression levels were compared at different stages of bladder cancer. To confirm the results on protein levels, we used immunohistochemistry on tissue microarrays of the same samples.
Results |
The expression of the p63 gene studied was significantly deregulated, with decreasing levels in early cancer versus normal tissue. Immunohistochemistry, performed on the same samples, using p63 antibody, confirmed the results of reverse transcriptase-polymerase chain reaction.
Conclusions |
The results of this study highlight that among the genes strongly deregulated in urothelial carcinoma, p63 is already abnormally expressed in the early stages.
Le texte complet de cet article est disponible en PDF.Plan
| This work was supported by the Société Française de Pathologie. |
Vol 70 - N° 3
P. 459-462 - septembre 2007 Retour au numéro
Article précédent
- Outcomes and Quality of Life of Adults Undergoing Continent Catheterizable Vesicostomy for Neurogenic Bladder
- Naji J. Touma, David Horovitz, Akshay Shetty, Yves Caumartin, Jorge De Maria, Patrick P.W. Luke
- Intravesical Botulinum Toxin A Injections Plus Hydrodistension Can Reduce Nerve Growth Factor Production and Control Bladder Pain in Interstitial Cystitis
- Hsin-Tzu Liu, Hann-Chorng Kuo
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?