Evaluating RNA Preparation Options for Archived Myocardial Biopsies - 11/08/11
Corresponding author. Tel.: +61 3 8344 5853; fax: +61 3 8344 5897.Résumé |
High quality RNA is the key to producing meaningful gene expression analyses. Human cardiac tissue specimens are extremely valuable, but may not always be obtained under optimal conditions and are frequently fibrotic. We provide a practical guide to assist in assessing the efficacy of two different RNA extraction methods applied to these challenging specimens. We describe how to compare ‘single-step’ and ‘multi-step’ extraction processes and discuss how to interpret information available through microfluidic and spectroscopic analyses to evaluate sample quality.
Le texte complet de cet article est disponible en PDF.Keywords : RNA, Isolation and purification, Organic extraction, Solid phase extraction, Human, Myocardium, Polymerase chain reaction (qRT-PCR)
Plan
Vol 20 - N° 5
P. 329-331 - mai 2011 Retour au numéro
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