Gelsolin down-regulates lipopolysaccharide-induced intraamniotic tumor necrosis factor-⍺ production in the midtrimester of pregnancy - 12/08/11
Address correspondence to Steven S. Witkin, PhD, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, 525 E 68 St, Box 35, New York, NY 10021Résumé |
Objective |
The purpose of this study was to identify gelsolin in midtrimester amniotic fluid and evaluate its interaction with lipopolysaccharide (LPS).
Study Design |
Supernatants from 40 midtrimester amniotic fluid samples were incubated with Escherichia coli LPS, and gelsolin binding was measured by enzyme-linked immunosorbent assay. Unfractionated aliquots of 25 of the fluids were cultured ex vivo for 24 hours in the presence of LPS and supernatants tested for tumor necrosis factor (TNF)-⍺ and interleukin (IL)-10 production, and the influence of antigelsolin antibody was evaluated.
Results |
Each amniotic fluid was positive for gelsolin that bound to LPS. LPS-induced TNF-⍺ production was inversely proportional to the amniotic fluid concentrations of LPS-bound gelsolin (r = -0.5047; P = .006). Preincubation with monoclonal antibody to gelsolin led to an increase in LPS-induced TNF-⍺ production (P = .01). There was no relationship between gelsolin and IL-10 production.
Conclusion |
Gelsolin is present in midtrimester amniotic fluid, binds to LPS, and inhibits the induction of TNF-⍺.
Le texte complet de cet article est disponible en PDF.Key words : gesolin, lipopolysaccharide, tumor necrosis factor-⍺, amniotic fluid
Plan
| Cite this article as: Sezen D, Bongiovanni AM, Gelber S, et al. Gelsolin down-regulates lipopolysaccharide-induced intraamniotic tumor necrosis factor-⍺ production in the midtrimester of pregnancy. Am J Obstet Gynecol 2009;200:191.e1-191.e4. |
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| Reprints not available from the authors. |
Vol 200 - N° 2
P. 191.e1-191.e4 - février 2009 Retour au numéro
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