CD203c expression on human basophils is associated with asthma exacerbation - 13/08/11
, Masami Taniguchi, MD, PhD a, Noritaka Higashi, MD, PhD a, Haruhisa Mita, PhD a, Keiichi Kajiwara a, Hiromichi Yamaguchi, MD a, Sayaka Tatsuno, MD a, Yuma Fukutomi, MD a, Hidenori Tanimoto, MD a, Kiyoshi Sekiya, MD a, Chiyako Oshikata, MD a, Takahiro Tsuburai, MD, PhD a, Naomi Tsurikisawa, MD, PhD a, Mamoru Otomo, MD, PhD a, Yuji Maeda, MD a, Maki Hasegawa, MD, PhD a, Eishi Miyazaki, MD, PhD b, Toshihide Kumamoto, MD, PhD b, Kazuo Akiyama, MD a
Reprint requests: Emiko Ono, MD, PhD, Division of Pulmonary Disease, Third Department of Internal Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Yufu, Oita 879-5593, Japan.Abstract |
Background |
CD203c is a basophil cell surface marker used to diagnose and monitor various allergic diseases, but its relationship to asthma is not clear.
Objective |
We determined whether CD203c expression levels are associated with stable and exacerbated asthma.
Methods |
We used flow cytometry to compare spontaneous expression levels of surface markers on basophils from patients with stable or exacerbated asthma and from healthy subjects. Longitudinal changes in these expression levels were measured after basophil stimulation by IgE-dependent or IgE-independent mechanisms and compared with patients’ asthma status.
Results |
Spontaneous expression levels of CD203c were significantly higher on basophils from patients with asthma exacerbation than patients with stable asthma or healthy subjects. In contrast, no differences in spontaneous expression levels of CD63 or CD69 were observed among the 3 groups. Anti-IgE–induced expression of CD203c significantly increased in basophils during asthma exacerbation (P = .005). Low concentrations of Dermatophagoides pteronyssinus or IL-3 induced higher expression levels of CD203c during asthma exacerbation than during clinical improvement; induction of CD203c expression by these antigens therefore correlates with asthma control. In the patients with clinical improvement, there was a correlation between spontaneous CD203c expression levels and the percent predicted values of FEV1 (r = -0.761; P = .022).
Conclusion |
Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission. Basophil expression levels of CD203c might therefore be used to monitor asthma in patients.
Le texte complet de cet article est disponible en PDF.Key words : Airway inflammation, asthma exacerbation, basophil activation, basophil surface marker, biomarker, CD203c
Abbreviations used : ENO, MFI, PEF, PGD2, 15R-MePGD2
Plan
| Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest. |
Vol 125 - N° 2
P. 483 - février 2010 Retour au numéro
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