Distinct pathophysiologic pathways induced by in vitro infection and cigarette smoke in normal human fetal membranes - 13/08/11
, Stephen J. Fortunato, MD a 
Reprints: Ramkumar Menon, PhD, The Perinatal Research Center, Centennial Women's Hospital, 2300 Patterson St, Nashville, TN 37203Résumé |
Objective |
The purpose of this study was to document distinct pathways that are initiated by lipopolysaccharide and cigarette smoke stimulation of normal term fetal membranes.
Study Design |
Fetal membranes from nonsmoking women at term, not in labor, from cesarean deliveries were placed in an organ explant system and stimulated with cigarette smoke extracts (CSEs), lipopolysaccharide, or lipopolysaccharide + CSE. Media were assayed for an interleukin (IL)-1β, -1 receptor antagonist, -6, -8, -10, tumor necrosis factor ⍺, soluble tumor necrosis factor receptors 1 and 2, and matrix metalloproteinases 1, 2, 3, 8, 9, and 12. Tissue homogenates were assayed for apoptotic markers (p53, caspase 3 activity, and cleaved poly [ADP-ribose] polymerase-1).
Results |
Lipopolysaccharide stimulation resulted in higher cytokine and matrix metalloproteinase concentrations, whereas it was lower after CSE and CSE + lipopolysaccharide stimulations, compared with control specimens. Apoptotic factors were several folds higher after CSE or CSE + lipopolysaccharide stimulation, compared with control specimens or lipopolysaccharide stimulations.
Conclusion |
Cigarette smoke showed immunoinhibitory properties that potentially were mediated by apoptosis and lipopolysaccharide-induced proinflammatory response. This study demonstrated 2 independent pathophysiologic pathways that may alter pregnancy outcome.
Le texte complet de cet article est disponible en PDF.Key words : behavioral factor, cigarette smoke, cytokine, fetal membrane, pPROM, toxin
Plan
| Cite this article as: Menon R, Fortunato SJ. Distinct pathophysiologic pathways induced by in vitro infection and cigarette smoke in normal human fetal membranes. Am J Obstet Gynecol 2009;200:334.e1-334.e8. |
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| Authorship and contribution to the manuscript is limited to the 2 authors indicated. There was no outside funding or technical assistance with the production of this article. |
Vol 200 - N° 3
P. 334.e1-334.e8 - mars 2009 Retour au numéro
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