Why eicosanoids could represent a new class of tocolytics on uterine activity in pregnant women - 13/08/11
Reprints: Jean-Charles Pasquier, MD, PhD, Obstetrics and Gynecology, CRC E-Lebel - CHUS, Université de Sherbrooke, 3001 12th Ave. North, J1H 5N4, Sherbrooke, QC, CanadaRésumé |
Objective |
The purpose of this study was to assess the effects of exogenous eicosanoids on spontaneous uterine contractile activity.
Study Design |
Eight uterine biopsies were performed from women who were undergoing elective cesarean delivery. Tension measurements were performed in vitro on myometrial strips. Contractile activities were quantified by the calculation of the area under the curve. The effects of eicosanoids and specific enzyme inhibitors were assessed. Fractions from various uterine tissues were analyzed by Western blot.
Results |
Data demonstrate the presence, in some tested tissues, of cytochrome P-450 epoxygenase and soluble epoxide hydrolase, which respectively produce and degrade epoxyeicosatrienoic acid regioisomers. Inhibition of soluble epoxide hydrolase with 12-(3-adamantan-1-yl-ureido)-dodecanoic acid or ω-hydroxylase with N-methylsulfonyl-12,12-dibromododec-11-enamide resulted in a tocolytic effect; N-methylsulfonyl-6-[2-propargyloxyphenyl] hexanamide, which is an epoxygenase inhibitor, had no effect. Exogenous epoxyeicosatrienoic acids displayed significant tocolytic effects on spontaneous contractile activities.
Conclusion |
Epoxy- and hydroxyeicosanoids represent new bioactive, arachidonic acid by-products with in vitro tocolytic activities. These findings suggest that cytochrome P-450 isozymes may represent relevant pharmacologic targets under physiopathologic conditions.
Le texte complet de cet article est disponible en PDF.Key words : CYP450-2J2, epoxyeicosatrienoic acid, epoxygenase, soluble epoxide hydrolase, tocolytic effect
Plan
| This work was supported by a Grant from the Fondation des Étoiles (J.C.P.) and a summer studentship from the Fonds de la recherche en santé du Québec (S.C.). |
|
| Cite this article as: Corriveau S, Berthiaume M, Rousseau E, et al. Why eicosanoids could represent a new class of tocolytics on uterine activity in pregnant women. Am J Obstet Gynecol 2009;201:420.e1-7. |
Vol 201 - N° 4
P. 420.e1-420.e7 - octobre 2009 Retour au numéro
Article précédent
- Incidence of complications in twin-twin transfusion syndrome after selective fetoscopic laser photocoagulation: a single-center experience
- Mounira Habli, Annette Bombrys, David Lewis, Foong-Yen Lim, William Polzin, Rose Maxwell, Timothy Crombleholme
- Racial and ethnic disparities in the trends in primary cesarean delivery based on indications
- Darios Getahun, Daniel Strickland, Jean M. Lawrence, Michael J. Fassett, Corinna Koebnick, Steven J. Jacobsen
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?