Estrogen receptor-beta mediates cyclooxygenase-2 expression and vascular prostanoid levels in human placental villous endothelial cells - 13/08/11
Reprints: Serdar E. Bulun, 303 E. Superior St., Lurie Building, 4-250, Chicago, IL 60611Résumé |
Objective |
Regulation of fetoplacental blood flow is likely mediated by factors such as prostanoids. Estrogen and its receptors affect prostanoid biosynthesis. Previously, we demonstrated that villous endothelial cells express estrogen receptor-beta (ESR2), and we sought to determine its role in the mediation of fetoplacental vascular function.
Study Design |
Villous endothelial cells from uncomplicated pregnancies were isolated, cultured, and treated with estrogen. RNA interference, real-time polymerase chain reaction, Western blotting, and enzyme immunoassays were performed.
Results |
Cyclooxygenase-2 (COX-2) expression levels were not altered consistently by estrogen. RNA interference of ESR2 led to a concomitant decrease in COX-2 messenger RNA (P < .0001) and protein (P < .05) in the presence and absence of estradiol. ESR2 knock-down also led to diminished prostacyclin and thromboxane concentrations in the absence of estradiol (P < .005).
Conclusion |
ESR2 mediates COX-2 expression levels and both prostacyclin and thromboxane concentrations in the basal state, which suggests the possibility of ligand-independent regulation of COX-2 activity and prostaglandin H2 substrate availability. Further investigation regarding ESR2 regulation of prostanoid biosynthesis and its effects on the fetoplacental vasculature is warranted.
Le texte complet de cet article est disponible en PDF.Key words : cyclooxygenase-2, estrogen receptor-beta, fetoplacental, villous endothelial cell
Plan
| Support for this research was provided by the AAOGF/SMFM Scholarship Award 2007-10 and Northwestern Memorial Foundation Private Donor Grant 2007-08. |
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| Cite this article as: Su EJ, Lin Z-H, Zeine R, et al. Estrogen receptor-beta mediates cyclooxygenase-2 expression and vascular prostanoid levels in human placental villous endothelial cells. Am J Obstet Gyncol 2009;200:427.e1-427.e8. |
Vol 200 - N° 4
P. 427.e1-427.e8 - avril 2009 Retour au numéro
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