Polyethyleneterephthalate Provides Superior Retention of Endothelial Cells During Shear Stress Compared to Polytetrafluoroethylene and Pericardium - 16/08/11
, Melissa Sgarioto, Bsc (Hons) a, Amal A. Owida, MEng a, William Yang, PhD b, Franklin L. Rosenfeldt, MD, FRACS c, Yos S. Morsi, PhD a
Corresponding author. Tel.: +61 3 9 214 5037; fax: +61 3 9 214 5050.Résumé |
Background |
Polyethyleneterephthalate (PET) and polytetrafluoroethylene (PTFE) are polymers successfully used as large diameter arterial grafts for peripheral vascular surgery. However, these prosthetic grafts are rarely used for coronary bypass surgery because of their low patency rates. Endothelialisation of the lumenal surface of these materials may improve their patency. This study aimed to compare the endothelialisation of PET, PTFE and pericardium by examining their seeding efficiency over time and the effect of various shear stresses on retention of endothelial cells.
Methods |
Ovine endothelial cells at 4×105cells/cm2 were seeded onto PET, PTFE and pericardium, and cultured for 1–168 hours. Cell coverage was determined via en face immunocytochemistry and cell retention was quantified after being subjected to shear stresses ranging from 0.018 to 0.037N/m2 for 15, 30 and 60 minutes.
Results |
Endothelial cells adhered to all of the materials one hour post-seeding. PET exhibited better cell retention rate, ranging from 66.9±5.6% at 0.018N/m2 for 15min to 44.7±1.9% at 0.037N/m2 for 60 minutes, when compared to PTFE and pericardium (p<0.0001, three-way ANOVA).
Conclusion |
PET shows superior retention of endothelial cells during shear stress compare to PTFE and pericardium.
Le texte complet de cet article est disponible en PDF.Keywords : Endothelial cells, Shear stress, PET, PTFE, Pericardium, Cell retention
Plan
Vol 15 - N° 6
P. 371-377 - décembre 2006 Retour au numéro
Article précédent
- Cardiovascular Magnetic Resonance Imaging for the Assessment of Right Heart Involvement in Cardiac and Pulmonary Disease
- Constantin B. Marcu, Aernout M. Beek, Albert C. Van Rossum
- Serial Angiography in Patients with Acute Coronary Syndromes: Effect of Antithrombotic Therapy on Angiographic Lesion Severity
- Ananth M. Prasan, Rohan Bhagwandeen, Victor J. Solanki, S. Benjamin Freedman, David Brieger
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?