First-trimester trophoblast cell model gene response to hypoxia - 18/08/11
, Jerome C. Nwachukwu, BA b, S. Joseph Huang, MD, PhD c, Seth Guller, PhD c, Ksenia Karpisheva, PhD b, Michael Garabedian, PhD b, Men-Jean Lee, MD c
Reprint requests: Nikki Koklanaris, MD, 550 First Ave, NBV-9N27, New York, NY 10016.Abstract |
Objective |
Trophoblast invasion, which sets the stage for placentation and pregnancy outcome, likely occurs in a hypoxic environment. We used microarray technology in a trophoblast cell line to identify hypoxia-responsive genes that may impact placentation.
Study design |
An immortalized extravillous cytotrophoblast cell line, HTR-8/SVneo, was exposed to normoxia (20% oxygen) or hypoxia (1% oxygen) for 6 hours. Total RNA was harvested and prepared for microarray study. Quantitative reverse transcriptase polymerase chain reaction was performed for array confirmation.
Results |
We confirmed the up- and down-regulation of 10 hypoxia-responsive genes using quantitative reverse transcriptase polymerase chain reaction. Ontologic gene categories that were found to be hypoxia-responsive included motility/migration, angiogenesis, and apoptosis.
Conclusion |
Specific genes that were found to be up-regulated in this first-trimester array (such as plasminogen activator inhibitor-1 and tissue inhibitor of metalloproteinase 3) have been described in preeclampsia. The hypoxia-responsive genes that we identified may be physiologic in early pregnancy. However, up-regulation of these same genes in later pregnancy augurs poorly.
Le texte complet de cet article est disponible en PDF.Key words : Microarray, Trophoblast invasion, Placenta, Hypoxia
Plan
| Presented at the 26th Annual Meeting of the Society for Maternal Fetal Medicine, January 30-February 4, 2006, Miami, FL. |
Vol 194 - N° 3
P. 687-693 - mars 2006 Retour au numéro
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