The expression and function of the endothelin system in contractile properties of vaginal myofibroblasts of women with uterovaginal prolapse - 18/08/11
Reprint requests: Lucienne Juillerat-Jeanneret, PhD, PD, MER, University Institute of Pathology, Bugnon 25, CH-1011 Lausanne, Switzerland.Abstract |
Objective |
The endothelin-1 system regulates (myo)fibroblast contraction in wound healing. Our aim was to determine endothelin-1 system expression and function in contractile properties of vaginal myofibroblasts of women with uterovaginal prolapse.
Study design |
Cultures of ⍺-smooth muscle actin-positive myofibroblasts that were established at the time of repair surgery for prolapse (n=30; mean age, 56 ± 14 years) were analyzed and compared for their expression of the endothelin-1 system and contractile properties to myofibroblasts from primiparous women.
Results |
Myofibroblasts expressed the complete endothelin system but did not secrete endothelin-1. Endothelin-1 binding was mediated exclusively by the endothelin B-receptor. In 3-dimensional collagen gels, spontaneous contraction of myofibroblasts from estrogen-treated women with prolapse was statistically significantly lower than from young primiparous women. Exogenous addition of endothelin-1 decreased the spontaneous contraction of myofibroblasts.
Conclusion |
Genital myofibroblasts of women with uterovaginal prolapse are poorly contractile, and endothelin-1 further decreases vaginal myofibroblast contraction, which is opposite to observations in skin myofibroblasts.
Le texte complet de cet article est disponible en PDF.Key words : Endothelin, Vaginal myofibroblast, Contraction, Prolapse
Plan
| Supported by grants from the Swiss National Science Foundation for Scientific Research (grants 20-52490.97, 3152-059219.99 and 3200.64907.01), the Swiss League and Research against Cancer (grants KFS 947-09-1999 and SKL 107-09.00), the Novartis Foundation for Scientific Research, and a Leenaards Foundation Award (Encouragement de la Recherche Scientifique). |
Vol 192 - N° 2
P. 426-432 - février 2005 Retour au numéro
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