A nonneuronal 5-hydroxytryptamine receptor 3 induces chloride secretion in the rat distal colonic mucosa - 18/08/11
Corresponding author. Tel.: +1-804-828-9513; fax: +1-804-827-0670.Abstract |
Background |
The 5-HT3 receptor is a serotonin receptor believed to reside on enteric neurons. However, several studies belie an exclusive neural localization. Our hypothesis is that the 5-HT3 receptor agonist, 2-methyl-5-HT (2Me5HT), induces chloride secretion despite neural blockade, which can be blocked by a 5-HT3 receptor antagonist.
Methods |
Rat distal colon was stripped of its muscularis, mounted as mucosal sheets in Ussing chambers, and short-circuited. Adjacent sheets were pretreated with 1 μmol/L of the neurotoxin, tetrodotoxin, and incubated with 2Me5HT (50 μmol/L) alone or with a 5-HT3 (0.3 μmol/L ondansetron or 0.3 μmol/L tropisetron) or a 5-HT4 (0.3 μmol/L GR11808) receptor antagonist. Short-circuit current (Isc) was measured continuously.
Results |
2Me5HT caused an increase in Isc, which was significantly (P <.01, repeated measures analysis of variance) inhibited by ondansetron (n = 8) and tropisetron (n = 5) but not by GR11808.
Conclusions |
A 5-HT3 receptor is present at the mucosal level that mediates chloride secretion by a nonneural pathway.
Le texte complet de cet article est disponible en PDF.Keywords : 5-hydroxytryptamine receptor 3, Intestinal secretion, Serotonin
Plan
Vol 190 - N° 5
P. 736-738 - novembre 2005 Retour au numéro
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