N-acetyl-cysteine (NAC) attenuates LPS-induced maternal and amniotic fluid oxidative stress and inflammatory responses in the preterm gestation - 19/08/11

Doi : 10.1016/j.ajog.2011.01.030

Nibal Awad, MD ^a, Nizar Khatib, MD ^a, Yuval Ginsberg, MD ^a, Zeev Weiner, MD ^a, Nir Maravi, MD ^a, Israel Thaler, MD ^a, Michael G. Ross, MD, MPH ^c, Joseph Itsokovitz-Eldor, MD ^a, Ron Beloosesky, MD ^a,^b,^⁎
^a Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel
^b Department of Obstetrics and Gynecology, Los Angeles, CA
^c Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA

Reprints: Ron Beloosesky, MD, 37 Borchov St., Ranana, Israel 43433

Résumé

Objective

Maternal infection is associated with oxidative stress and inflammation. We sought to determine whether N-acetyl-cysteine can decrease maternal oxidative stress and the inflammatory response in preterm gestation.

Study Design

Pregnant rats 16 days, were treated with (1) lipopolysaccharide, (2) N-acetyl-cysteine 120 minutes after lipopolysaccharide, or (3) saline solution (intraperitoneal). Six hours after lipopolysaccharide administration, serum lipid peroxide formation (LPO), tumor necrosis factor-⍺, interleukin-6, and interleukin-1β levels in maternal serum and amniotic fluid were determined.

Results

Lipopolysaccharide significantly increased maternal serum lipid peroxide formation (24-118.5 nmol/mL; P < .05), and maternal serum and amniotic fluid tumor necrosis factor-⍺, interleukin-6, and interleukin-1β. N-acetyl-cysteine treatment after lipopolysaccharide significantly attenuated lipid peroxide formation (47.5 nmol/mL) and proinflammatory cytokines response in maternal serum and amniotic fluid.

Conclusion

Maternal and amniotic fluid oxidative stress and inflammatory stimulation are attenuated by N-acetyl-cysteine even when administered after lipopolysaccharide. These results suggest that N-acetyl-cysteine may protect the fetus from adverse sequelae associated with inflammatory stimulation.

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Key words : amniotic fluid, inflammation, maternal infection, N-acetyl-cysteine, rat

Plan

Materials and Methods

Animals and treatments

Sample collection

Enzyme-linked immunoabsorbant assay determinations

	Support was provided by The Legacy Heritage Clinical Research Initiative of the Israel Science Foundation (Grant no. 1814/07).
	Cite this article as: Awad N, Khatib N, Ginsberg Y, et al. N-acetyl-cysteine (NAC) attenuates LPS-induced maternal and amniotic fluid oxidative stress and inflammatory responses in the preterm gestation. Am J Obstet Gynecol 2011;204:450.e15-20.

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Vol 204 - N° 5

P. 450.e15-450.e20 - mai 2011 Retour au numéro

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N-acetyl-cysteine (NAC) attenuates LPS-induced maternal and amniotic fluid oxidative stress and inflammatory responses in the preterm gestation - 19/08/11

Résumé

Objective

Study Design

Results

Conclusion

Plan

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