Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis - 19/08/11
Résumé |
Objective |
The objective was to examine the use of a tailor-made DNA microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis.
Study Design |
One hundred one women attending a health center for HIV testing in South Africa were enrolled. Stained, liquid-based cytology slides were scored for bacterial vaginosis. An inventory of organisms was obtained using microarray technology, probing genera associated with bacterial vaginosis in more detail, namely Gardnerella, Atopobium, Dialister, Leptotrichia, Megasphaera, Mobiluncus, Peptostreptococcus, Prevotella, and Sneathia.
Results |
Of 101 women, 34 were diagnosed positive for bacterial vaginosis. This condition was associated with an increased microbial diversity. It is no longer useful to base the diagnosis of bacterial vaginosis on Gardnerella alone. Rather, its presence with Leptotrichia and Prevotella species, and especially Atopobium was more indicative of an aberrant state of the vaginal flora.
Conclusion |
To understand the vaginal microbiota in more detail, microarray-based identification can be used after microscopic scoring.
Le texte complet de cet article est disponible en PDF.Key words : bacterial vaginosis, microarray, microscopy, South-African, vaginal microbiota
Plan
| Reprints not available from the authors. |
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| Leiden Cytology and Pathology Laboratory (LCPL) is an independent laboratory, its main occupation is to analyze samples of screening programs for cervical carcinoma in the Netherlands and develop better screening methods. In collaboration with TNO, the microarray was developed. No conflicts of interest were present, either at LCPL, TNO, Erasmus University, Lawson Research Institute or Bontekoe Research. |
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| Cite this article as: Dols JAM, Smit PW, Kort R, et al. Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis. Am J Obtet Gynecol 2011;204:305.e1-7. |
Vol 204 - N° 4
P. 305.e1-305.e7 - avril 2011 Retour au numéro
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