A correlation between polyomavirus JC virus quantification and genotypes in renal transplantation - 19/08/11
, Ming-Chi Lu, M.D. b, c, Ming-Che Lee, M.D. d, b, Su-Chin Liu, M.S. e, Teng-Yi Lin, M.S. f, Ning-Sheng Lai, Ph.D. b, c, ⁎
Corresponding author: Tel.: + 886-5-2648000; fax: +886-5-2648006Abstract |
Objective |
To determine whether the John Cunningham virus (JCV) viral load and the multigenotypes in viruria are correlated with transplant patients.
Methods |
The urine of 60 renal transplant patients and 60 healthy controls were screened. We used quantitative real-time polymerase chain reaction and capillary electrophoresis to assess viral load and genotype respectively.
Results |
The incidence of viruria and viral load were higher in transplant patients with P = .0092 and P = .0094, respectively. The incidence of different genotype in transplant patients versus controls was 8.3% versus 13.3% for single genotype, 26.7% versus 5% for 2 genotypes, and 5% versus 0% for multigenotypes (P = .0004). The incidence of more than 2 genotypes was high in people with a high viral load and closely related with the transplant patients (P = .007).
Conclusions |
Not only viral load but also genotypes are important as a screening parameter to understand the immune milieu of the patients to prevent subsequent complications like polyomavirus nephropathy, infection, and malignancy.
Le texte complet de cet article est disponible en PDF.Keywords : Capillary electrophoresis, JC virus, Polyomavirus, Real-time polymerase chain reaction, Transplant patients
Plan
Vol 200 - N° 1
P. 53-58 - juillet 2010 Retour au numéro
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