Magnesium sulfate reduces inflammation-associated brain injury in fetal mice - 19/08/11
, Kelsey Breen, BS, Alexander Friedman, MD, MPH, Jinghua Chai, MD, Michal A. Elovitz, MD
Reprints: Irina D. Burd, MD, PhD, Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania, 1353 Biomedical Research Bldg. II/III, 421 Curie Blvd., Philadelphia, PA 19104-6142Résumé |
Objective |
The purpose of this study was to investigate whether magnesium sulfate (MgSO4) prevents fetal brain injury in inflammation-associated preterm birth (PTB).
Study Design |
Using a mouse model of PTB, lipopolysaccharide (LPS) or normal saline solution (NS)–exposed mice were randomized to intraperitoneal treatment with MgSO4 or NS by intrauterine injection. From the 4 treatment groups (NS + NS; LPS + NS; LPS + MgSO4; and NS + MgSO4), fetal brains were collected for quantitative polymerase chain reaction studies and primary neuronal cultures. Messenger RNA expression of cytokines, cell death, and markers of neuronal and glial differentiation were assessed. Immunocytochemistry and confocal microscopy were performed.
Results |
There was no difference between the LPS + NS and LPS + MgSO4 groups in the expression of proinflammatory cytokines, cell death markers, and markers of prooligodendrocyte and astrocyte development (P > .05 for all). Neuronal cultures from the LPS + NS group demonstrated morphologic changes; this neuronal injury was prevented by MgSO4 (P < .001).
Conclusion |
Amelioration of neuronal injury in inflammation-associated PTB may be a key mechanism by which MgSO4 prevents cerebral palsy.
Le texte complet de cet article est disponible en PDF.Key words : inflammation, magnesium sulfate, mouse model, neuronal injury, preterm birth
Plan
| Cite this article as: Burd I, Breen K, Friedman A, et al. Magnesium sulfate reduces inflammation-associated brain injury in fetal mice. Am J Obstet Gynecol 2010;202:292.e1-9. |
|
| Supported by National Institutes of Health Grant 5-R01-HD046544-0 (M.A.E.) and in part by the ABOG/AAOGF scholarship (I.B.). |
Vol 202 - N° 3
P. 292.e1-292.e9 - mars 2010 Retour au numéro
Article précédent
- Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice
- Dong Zhang, Mohamed Al-Hendy, Gloria Richard-Davis, Valerie Montgomery-Rice, Chakradhari Sharan, Veera Rajaratnam, Anjali Khurana, Ayman Al-Hendy
- Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes
- C. Bettina Portmann-Lanz, Andreina Schoeberlein, Reto Portmann, Stefan Mohr, Pierre Rollini, Ruth Sager, Daniel V. Surbek
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?