Preimplantation factor promotes first trimester trophoblast invasion - 19/08/11
, Eytan R. Barnea, MD b, c, Min Li, PhD d, S. Joseph Huang, MD, PhD d, Graciela Krikun, PhD d, Michael J. Paidas, MD a
Reprints: Christina M. Duzyj, MD MPH, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 333 Cedar St., FMB 339B, New Haven, CT 06520-8063Résumé |
Objective |
Preimplantation factor is a novel embryo-derived peptide that influences key processes in early pregnancy implantation, including immunity, adhesion, remodeling, and apoptosis. Herein, we explore the effects of synthetic preimplantation factor on trophoblast invasion.
Study Design |
Invasion patterns of immortalized cultured HTR-8 trophoblast cells were analyzed through Matrigel extracellular matrix ± synthetic preimplantation factor (25-100 nM) in a transwell assay. Effects were compared with epidermal growth factor 10 μg/mL, scrambled aminoacid sequence of preimplantation factor, or media alone as controls.
Results |
Synthetic preimplantation factor enhances trophoblast invasion at physiologic doses (at 50 nM, 260%; 95% confidence interval [CI], 174–346%; P = .05; 100 nM ,178%; 95% CI, 170–184%; P < .02), compared with scrambled amnioacid sequence preimplantation factor or control media. Epidermal growth factor added to synthetic preimplantation factor does not further enhance trophoblast invasion (synthetic preimplantation factor 50 nM + epidermal growth factor, 238%; 95% CI, 237–239%; P < .03; synthetic preimplantation factor 100 nM + epidermal growth factor 269%; 95% CI, 265–273%; P < .04).
Conclusion |
Preimplantation factor should be further investigated as it shows a potential preventative or therapeutic role for pregnancy complications associated with inadequate trophoblast invasion.
Le texte complet de cet article est disponible en PDF.Key words : first trimester trophoblast, preimplantation factor, trophoblast invasion
Plan
| Research conducted at Yale University, New Haven, CT, supported by BioIncept, LLC (M.J.P.), and NIH Grant no. 5R01HD056123-02 (S.J.H.). |
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| Cite this article as: Duzyj CM, Barnea ER, Li M, et al. Preimplantation factor promotes first trimester trophoblast invasion. Am J Obstet Gynecol 2010;203:402.e1-4. |
Vol 203 - N° 4
P. 402.e1-402.e4 - octobre 2010 Retour au numéro
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