Does sildenafil citrate affect myometrial contractile response to nifedipine in vitro? - 19/08/11
, Maged M. Costantine, MD, Ancizar Betancourt, MS, Gary D.V. Hankins, MD, Monica Longo, MD, PhD, George R. Saade, MD, Egle Bytautiene, MD, PhD
Reprints: Giuseppe Chiossi, MD, Department of Obstetrics and Gynecology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0144Résumé |
Objective |
We sought to test the hypothesis that sildenafil citrate (SC) at low concentrations potentiates the tocolytic effects of nifedipine in vitro.
Study Design |
Myometrial biopsies were obtained from 22 term nonlaboring women undergoing scheduled cesarean delivery. Tissue strips were suspended in organ chambers for isometric tension recording, and incubated for 30 minutes with either SC at 231 ng/mL or solvent. The effects of cumulative doses (10–10 to 10–5 mol/L) of nifedipine on spontaneous and oxytocin-induced uterine contractility were then determined. Areas under the contraction curve were compared using 1-way analysis of variance with Tukey post hoc test (significance: P < .05).
Results |
Nifedipine significantly inhibited spontaneous and oxytocin-induced myometrial contractility. Preincubation with SC increased response to nifedipine and significantly potentiated its inhibitory effect at 10–8 mol/L, without affecting oxytocin-induced contractile response.
Conclusion |
At concentrations within a therapeutic window, SC increases myometrial sensitivity to nifedipine.
Le texte complet de cet article est disponible en PDF.Key words : nifedipine, organ chamber, oxytocin, sildenafil citrate, uterine contractility
Plan
| Financial support was provided by the Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX. |
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| Cite this article as: Chiossi G, Costantine MM, Betancourt A, et al. Does sildenafil citrate affect myometrial contractile response to nifedipine in vitro? Am J Obstet Gynecol 2010;203:252.e1-5. |
Vol 203 - N° 3
P. null - septembre 2010 Retour au numéro
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