CD1d restricted natural killer T cells are not required for allergic skin inflammation - 20/08/11
, Muriel Pichavant, PhD , Rui He, PhD, Jordan Scott, MD, Everett Meyer, PhD, Sho Goya, MD, PhD, Raif S. Geha, MD ⁎, ‡ 
, Dale T. Umetsu, MD, PhD ‡Reprint requests: Raif S. Geha, MD, Karp Building, Division of Immunology, Children’s Hospital, One Blackfan Circle, Boston, MA 02115.Boston, Mass
Abstract |
Background |
Invariant T-cell receptor–positive natural killer (iNKT) cells have been shown to be essential for the development of allergen-induced airway hyperreactivity (AHR).
Objective |
We examined the role of iNKT cells in allergic skin inflammation using a murine model of atopic dermatitis (AD) elicited by epicutaneous sensitization with ovalbumin (OVA).
Methods |
Wild-type (WT) and natural killer T-cell–deficient CD1d–/– mice were epicutaneously sensitized with OVA or normal saline and challenged with aerosolized OVA. iNKT cells in skin and bronchoalveolar lavage fluid were analyzed by fluorescence-activated cell sorting, and cytokine mRNA levels were measured by quantitative RT-PCR. AHR to methacholine was measured after OVA inhalation.
Results |
Skin infiltration by eosinophils and CD4+ cells and expression of mRNA encoding IL-4 and IL-13 in OVA-sensitized skin were similar in WT and CD1d–/– mice. No significant increase in iNKT cells was detectable in epicutaneously sensitized skin. In contrast, iNKT cells were found in the bronchoalveolar lavage fluid from OVA-challenged epicutaneously sensitized WT mice, but not CD1d–/– mice. Epicutaneously sensitized CD1d–/– mice had an impaired expression of IL-4, IL-5, and IL-13 mRNA in the lung and failed to develop AHR in response to airway challenge with OVA.
Conclusion |
These results demonstrate that iNKT cells are not required for allergic skin inflammation in a murine model of AD, in contrast with airway inflammation, in which iNKT cells are essential.
Clinical implications |
Understanding the potential role of iNKT cells in AD will allow us to have a more specific target for therapeutic use.
Le texte complet de cet article est disponible en PDF.Key words : Atopic dermatitis, allergic skin inflammation, NKT cells, TH2 cytokines, eosinophils
Abbreviations used : AD, ⍺-GalCer, AHR, BAL, FACS, NK, NKT, OVA, TCR, WT
Plan
| Supported by National Institutes of Health grant AI-31541. Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest. |
Vol 118 - N° 6
P. 1363-1368 - décembre 2006 Retour au numéro
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