Group B streptococcus exploits lipid rafts and phosphoinositide 3-kinase/Akt signaling pathway to invade human endometrial cells - 21/08/11
Reprints: Chandra Yallampalli, DVM, PhD, Distinguished Professor, Department of Obstetrics and Gynecology, University of Texas Medical Branch, 301 University Blvd, MRB, 11.138, Rt 106,2 Galveston, TX 77555-1062Résumé |
Objective |
We sought to determine the role lipid rafts and phosphoinositide 3-kinase (PI3K) in invasiveness of group B streptococci (GBS) to endometrial cells.
Study Design |
Antibiotic protection assay and electron microscopy were used to evaluate the invasion of GBS to human endometrial Ishikawa cells cholesterol-depleted by using methyl-beta-cyclodextrin or treated with PI3K inhibitors: wortmannin or LY294002. Immunoblotting analysis of Akt phosphorylation and cellular imaging of GFP-Akt-PH probe were used to assess PI3Ks activation in infected cells.
Results |
Infected Ishikawa cells streptococci are associated to membrane ruffles with morphological features of undergoing internalization. GBS remained attached but completely failed to invade to cholesterol-depleted human endometrial cells or displayed decreased invasiveness in the presence of PI3K inhibitors. Cholesterol depletion resulted in loss of membrane ruffling and dispersion of raft-associated molecules: monosialoganglioside GM1 and PI3K.
Conclusion |
This work provides the evidence that lipid rafts and raft-associated PI3K are implicated in GBS invasion to human endometrial cells.
Le texte complet de cet article est disponible en PDF.Key words : group B streptococcus, PI3K, Ishikawa cells, kinase, lipid rafts
Plan
| This study was supported in part by Grant HL-72650 from the National Institutes of Health (to C.Y.). |
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| Cite this article as: Goluszko P, Popov V, Wen J, et al. Group B streptococcus exploits lipid rafts and phosphoinositide 3-kinase/Akt signaling pathway to invade human endometrial cells. Am J Obstet Gynecol 2008;199:548.e1-548.e9. |
Vol 199 - N° 5
P. null - novembre 2008 Retour au numéro
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