Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes - 22/08/11
, Beth L. Pineles, BS a, Adi L. Tarca, PhD a, c, Yeon Mee Kim, MD a, d, Sorin Draghici, PhD c, Juan Pedro Kusanovic, MD a, Jung-Sun Kim, MD, PhD a, d, Offer Erez, MD a, Shali Mazaki-Tovi, MD a, e, Sonia Hassan, MD a, e, Jimmy Espinoza, MD a, e, Chong Jai Kim, MD, PhD a, d, ⁎ 
Address correspondence to: Roberto Romero, MD, of Chong Jai Kim, MD, PhD, Perinatology Research Branch, NICHD, NIH, DHHS, Wayne State University/Hutzel Women’s Hospital, 3990 John R, Box 4, Detroit, MI 48201Résumé |
Objective |
The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation.
Study Design |
Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10).
Results |
More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories.
Conclusion |
Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.
Le texte complet de cet article est disponible en PDF.Key words : chorioamniotic membranes, gestation, inflammation, labor, microRNA, real-time qRT-PCR
Plan
| Supported by the Intramural Research Program of the National Institute of Child Health and Human Development, NIH, DHHS. Reprints not available from the authors. Cite this article as: Montenegro D, Romero R, Pineles BL, et al. Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes. Am J Obstet Gynecol 2007;197:289.e1-289.e6. |
Vol 197 - N° 3
P. 289.e1-289.e6 - septembre 2007 Retour au numéro
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