Evidence for multiple circulating factors in preeclampsia - 22/08/11
, Sarah Hart, PhD 2, Stuart Armstrong, BSc 3, Gary J. Mires, MD 4, Robert Beynon, PhD 3, Simon J. Gaskell, PhD 2, Philip N. Baker, MD 1
Reprints: Jenny Myers, PhD, Maternal & Fetal Health Research Centre, University of Manchester, St Mary’s Hospital, Hathersage Rd, Manchester, M13 0JH UKRésumé |
Objective |
The purpose of this study was to enrich vasoactive factors that are present in the plasma of women with preeclampsia by the application of sequential fractionation and determination of the activity of each fraction in a bioassay.
Study design |
Pooled plasma from women with preeclampsia (n = 23) and matched control subjects (n = 23) was subjected to fractionation with ultrafiltration, targeted immunodepletion, or size exclusion chromatography. Myometrial arteries that were isolated from healthy cesarean section biopsy specimens (n = 28) were incubated with plasma fractions (2%, volume/volume), and their endothelial function was assessed by wire myography.
Results |
Incubation of arteries with preeclampsia plasma or immunodepleted preeclampsia plasma had a deleterious effect on endothelial-dependent relaxation. Bioactivity of the plasma factors was absent in all fractions after either plasma ultrafiltration or separation with the use of size exclusion chromatography; however, activity was restored after recombination of these fractions.
Conclusion |
This study provides the first conclusive evidence that multiple synergistic factors, with a combined vasoactive effect, are present in the plasma of women with preeclampsia.
Le texte complet de cet article est disponible en PDF.Key words : endothelium, hypertension, plasma, preeclampsia, pregnancy, relaxation
Plan
| Supported by The British Heart Foundation (Grant Ref No. PG/03/040/15275) and Tommy’s, the baby charity. Cite this article as: Myers JE, Hart S, Armstrong S, et al. Evidence for multiple circulating factors in preeclampsia. Am J Obstet Gynecol 2007;196:266.e1-266.e6. |
Vol 196 - N° 3
P. 266.e1-266.e6 - mars 2007 Retour au numéro
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