Dose-dependent lipopolysaccharide-induced fetal brain injury in the guinea pig - 22/08/11
Reprints: Graeme N. Smith, MD, PhD, Department of Obstetrics and Gynecology, Queen’s University, Kingston General Hospital, 76 Stuart St, Kingston, Canada K7L 2V7Résumé |
Objective |
This study determined whether a lipopolysaccharide (LPS) dose-dependent increase in fetal brain injury occurs to further characterize the relationship between maternal inflammation and fetal brain injury.
Study Design |
Pregnant guinea pigs (n = 59) at 70% gestation were injected intraperitoneally with 1, 5, 25, 50, 100, 200, or 300 μg LPS per kilogram of maternal body weight or an equivalent volume of vehicle. Animals were killed 7 days later. Maternal serum and amniotic fluid samples were assayed for proinflammatory cytokines tumor necrosis factor-⍺, interleukin-1β, and interleukin-6 using enzyme-linked immunosorbent assay kits. Fetal brains (n = 72) were stained for evidence of cell death with NeuroTACS stain.
Results |
Seven days after LPS injections, cytokine concentrations in maternal serum and amniotic fluid were not different (P > .05) from controls. Levels of cell death in all brain regions examined were highest following the maternal administration of 300 μg/kg LPS (P < .05). The dose effect was brain region–dependent (P < .05).
Conclusion |
A threshold of maternal infection/inflammation exists, beyond which demonstrable fetal brain injury may result.
Le texte complet de cet article est disponible en PDF.Key words : brain injury, chorioamnionitis, fetal, guinea pig, lipopolysaccharide
Plan
| This work was supported in part by the Physicians of Ontario through a grant from the Physicians’ Services Incorporated Foundation (G.N.S.), an Ontario Graduate Scholarship (E.L.H.), and an R. Samuel McLaughlin Fellowship (E.L.H.). Additional funding for was obtained through a Canadian Institutes of Health Research New Investigators Award (G.N.S.). |
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| Cite this article as: Harnett EL, Dickinson MA, Smith GN. Dose-dependent lipopolysaccharide-induced fetal brain injury in the guinea pig. Am J Obstet Gynecol 2007;197:179.e1-179.e7. |
Vol 197 - N° 2
P. 179.e1-179.e7 - août 2007 Retour au numéro
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