Urinary eicosanoid and tyrosine derivative concentrations in patients with vasculitides - 24/08/11
, Haruhisa Mita, PhD a, Masami Taniguchi, MD, PhD a, Naomi Turikisawa, MD, PhD a, Ai Higashi, MD a, b, Yoshinori Ozawa, MD, PhD a, Shigeto Tohma, MD, PhD a, Kimiyoshi Arimura, MD, PhD b, Kazuo Akiyama, MD a
Reprint requests: Noritaka Higashi, MD, Scheeles väg1, PO Box 287, National Institute of Environmental Medicine, Karolinska Institutet, SE-171 77 Stockholm, Sweden.Sagamihara, Kanagawa, and Kagoshima, Japan
Abstract |
Background |
Vasculitides are classified on the basis of the type of cell involved, namely, eosinophilic vasculitides such as Churg-Strauss syndrome (CSS) and noneosinophilic vasculitides. However, knowledge on inflammatory mediators and oxidative tissue damage associated with vasculitides is insufficient.
Objective |
We measured the urinary concentrations of inflammatory mediators and tyrosine derivatives to assess biomarkers associated with the pathophysiology of vasculitides.
Methods |
Urine was collected from 9 patients with CSS during acute exacerbation and during clinical remission, 24 patients with rheumatoid arthritis in stable condition, and 8 patients with vasculitis diseases (VDs) during acute exacerbation. Leukotriene E4 (LTE4), 9⍺,11β prostaglandin F2, and eosinophil-derived neurotoxin (EDN) concentrations were determined by enzyme immunoassay. 3-Bromotyrosine (BrY) and 3-chlorotyrosine (ClY) concentrations were determined by gas chromatography-mass spectrometry.
Results |
The urinary LTE4, EDN, BrY, and ClY concentrations were significantly higher in the patients with CSS during acute exacerbation than in healthy control subjects and, except for urinary ClY concentration, significantly decreased during clinical remission. The urinary EDN and BrY concentrations were significantly higher in patients with CSS during acute exacerbation than in patients with VD during acute exacerbation. Only urinary LTE4 concentration was significantly different between the patients with rheumatoid arthritis in stable condition and the patients with VD during acute exacerbation.
Conclusion |
Oxidative tissue damage caused by eosinophil peroxidase is a pathophysiological characteristic of eosinophil-associated diseases such as CSS. Urinary LTE4 concentration may reflect a pathophysiological event involved in eosinophilic and noneosinophilic vasculitides. Cysteinyl-leukotriene pathways are potential therapeutic targets for small-vessel vasculitides.
Le texte complet de cet article est disponible en PDF.Key words : Churg-Strauss syndrome, vasculitides, 3-bromotyrosine, 3-chlorotyrosine, leukotriene E4
Abbreviations used : ANCA, BrY, ClY, cr, CSS, cysLT, EDN, EPO, HC, HOBr, LT, MPA, PG, RA, TA, VD, WG
Plan
Vol 114 - N° 6
P. 1353-1358 - décembre 2004 Retour au numéro
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