Insulin enhances the transcription of luteinizing hormone-β gene - 25/08/11
Abstract |
Objective |
Hyperinsulinemia and insulin resistance are implicated in the pathophysiology of polycystic ovary syndrome, a condition associated with elevated levels of LH. We tested the hypothesis that insulin enhances the transcriptional activity of LHβ promoter.
Study design |
We transfected the gonadotrope cell line LβT2 with a plasmid that expresses the proximal promoter of LHβ gene upstream of luciferase, and determined insulin effect on endogenous LHβ mRNA.
Results |
We found that insulin stimulated (2-4 fold) the activity of this promoter in a time- and concentration-dependent manner. Accordingly, insulin up-regulated the level of LHβ mRNA. In contrast, insulin had no significant effect on GnRH-dependent LHβ expression. The expression of Egr-1 and SF-1, which are essential for transcription of LHβ gene, was unchanged by insulin.
Conclusion |
Insulin enhances the transcription of LHβ gene. This modulation may contribute to the pathophysiology of polycystic ovary syndrome.
Le texte complet de cet article est disponible en PDF.Keywords : Luteinizing hormone, Insulin, Egr-1, Steroidogenic factor 1
Plan
 | Christoph Dorn's present address is the Department of Obstetrics and Gynecology, University of Bonn, Bonn, D-53105 Germany. Reprints not available from the authors. |
Vol 191 - N° 1
P. 132-137 - juillet 2004 Retour au numéro
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