Leukemia inhibitory factor and homeobox a10 gene expression in an ectopic pregnancy model for implantation - 25/08/11
Abstract |
Objective |
We investigated the expression of leukemia inhibitory factor and homeobox a10 in endometrium that was derived from patients with concurrent ectopic pregnancies compared with nonpregnant luteal phase endometrium from the same patients.
Study design |
Endometrial biopsy specimens that were obtained from women (n=3) who underwent surgery for ectopic pregnancy were matched with biopsy specimens in a nonpregnant luteal phase from the same patients. Quantitative reverse transcription polymerase chain reaction for leukemia inhibitory factor and homeobox a10 was performed. Polymerase chain reaction products were assayed with laser detection of nanogram quantities of complementary deoxyribonucleic acid. Ratios of each implantation marker to glyceraldehyde-3-phosphate dehydrogenase as an internal standard were compared for quantification.
Results |
Leukemia inhibitory factor expression is increased in endometrium from ectopic pregnancy; homeobox a10 is increased in luteal phase endometrium.
Conclusion |
Leukemia inhibitory factor appears to be regulated by human chorionic gonadotropin or other factors of pregnancy; homeobox a10 is regulated by ovarian steroids of the luteal phase.
Le texte complet de cet article est disponible en PDF.Keywords : Implantation, Leukemia inhibitory factor, Homeobox a10 gene, Ectopic pregnancy
Plan
| Supported in part by the Vicksburg Hospital Medical Foundation, Vicksburg, Miss. Reprints not available from the authors. |
Vol 190 - N° 5
P. 1419-1421 - mai 2004 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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