Minimizing red blood cell contamination while isolating mononuclear cells from whole blood: the next step for the treatment of severe hemolytic disease of the fetus/newborn - 28/08/11
Reprint requests: Kenneth J. Moise, Jr, MD, 214 MacNider Bldg, CB #7516, Chapel Hill, NC 27599.Chapel Hill, NC
Abstract |
Objective |
We examined whether mononuclear cell purification of human blood could be done while minimizing contamination with RhD-positive red blood cells to treat hemolytic disease of the fetus/newborn that was caused by rhesus disease.
Study design |
Whole blood from 16 individuals who tested rhesus positive was diluted and centrifuged over a Ficoll gradient. The cell pellet was incubated with red blood cell lysis buffer, divided into three samples, and analyzed for cell count, mononuclear cell yield, and RhD-positive red cell contamination by flow cytometry.
Results |
Mean RhD-positive red cell contamination was 0.24% (range, 0%-1.9%). The average yield of mononuclear cells was 11.5% (range, 1.8%-23.6%). Through regression analysis, 34 to180 mL of paternal whole blood would be necessary to achieve an antigen load that is sufficient for an HLA antibody response.
Conclusion |
Purification of human blood is possible to produce reasonable mononuclear cell yields with minimal rhesus activity, which makes paternal leukocyte therapy a plausible treatment for severe rhesus alloimmunization.
Le texte complet de cet article est disponible en PDF.Keywords : Paternal leukocyte therapy, monocytes, red blood cell alloimmunization, rhesus disease, hemolytic disease of the fetus/newborn
Plan
 | Presented at the Twenty-Third Annual Meeting of the Society for Maternal-Fetal Medicine, San Francisco, Calif, February 3-8, 2003. |
Vol 189 - N° 4
P. 1012-1016 - octobre 2003 Retour au numéro
Article précédent
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