A polymorphism in the promoter of the CD14 gene (CD14/-159) is associated with the development of coronary artery lesions in patients with Kawasaki disease - 29/08/11
, Megumi Hara, MD, Goro Yokota, MD, Mamie Watanabe, MD, Yoshiaki Ueda, MD, Miyoko Imayoshi, MD, Eiichi Ishii, MD, Hakaru Tasaki, MD, Yuhei Hamasaki, MD
Reprint requests: Masafumi Zaitsu, MD, Department of Pediatrics, Faculty of Medicine, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.Abstract |
Objective |
To investigate whether a polymorphism in the CD14 gene is associated with Kawasaki disease (KD).
Study design |
We extracted DNA from the whole blood of 69 control children and 67 patients with KD. We determined a polymorphism in the CD14 gene at position -159 upstream from the major transcription site (CD14/-159) by restriction fragment assay. We then investigated the association between CD14/-159 and the onset of KD and development of coronary artery lesion (CAL).
Results |
The genomic and allelic frequencies of the polymorphism were not different between normal children and KD patients. The KD patients with TT genotypes at CD14/-159 had more CAL complications than those with CT and CC (OR, 4.05; 95% CI, 1.34-12.22). The frequencies of the T allele was significantly higher than that of the C allele in KD patients with CAL (OR, 2.20; 95% CI, 1.23-3.94). Their data were confirmed in the patients whether the patients were treated with intravenous gamma-globulin. KD patients with TT genotypes had significantly higher levels of C-reactive protein and vascular endothelial growth factor, which had previously been reported as risk factors for CAL, than those with CC genotypes.
Conclusion |
These results indicate that the T allele and TT genotype at CD14/-159 are risk factors for CAL in KD, and that the development of CAL in KD may be related to the magnitude of CD14 toll-like receptor response.
Le texte complet de cet article est disponible en PDF.Abbreviations : AMI, CAD, CAL, CRP, DEPC, ELISA, HGF, INF, IL, IVGG, KD, LPS, PCR, TLR, TNF, VEGF, WBC
Plan
 | Supported by a grant from The Ministry of Education, Science, Sports, and Culture of Japan. |
Vol 143 - N° 3
P. 357-362 - septembre 2003 Retour au numéro
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