Limiting the proliferation and reactivity of retinal Müller cells during experimental retinal detachment: the value of oxygen supplementation - 08/09/11
Abstract |
PURPOSE: To assess the role of hypoxia in inducing the proliferation, hypertrophy, and dysfunction of Müller cells in detached retina and the effectiveness of supplemental oxygen in limiting these reactions.
METHODS: Retinal detachments were produced in the right eye of each of 13 cats; the cats survived surgery for 3 days, during which six were kept in normoxia (room air, 21%) and seven in hyperoxia (70% oxygen). Retinas were labeled for proliferation with an antibody (MIB-1) to a cell cycle protein (Ki-67), for evidence of hypertrophy employing antibodies to the intermediate filament protein glial fibrillary acidic protein (GFAP) and to β-tubulin and for disturbance of glutamate neurochemistry employing antibodies to glutamate to a glutamate receptor (GluR-2) and to glutamine synthetase.
RESULTS: Results from the two animals kept in normoxia after retinal detachment confirmed previous reports that detachment caused the proliferation of Müller cells, the hypertrophy of Müller cell processes, and the disruption of glutamate recycling by Müller cells. Oxygen supplementation during detachment reduced Müller cell proliferation and hypertrophy and reduced the abnormalities in the distributions of glutamate, GluR-2, and glutamine synthetase.
CONCLUSIONS: Oxygen supplementation reduced the reaction of retinal Müller cells to retinal detachment, limiting their proliferation and helping to maintain their normal structure and function. In the clinical setting, oxygen supplementation between diagnosis and reattachment surgery may reduce the incidence and severity of glial-based complications, such as proliferative vitreoretinopathy.
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This study was supported by the Australian Retinitis Pigmentosa Association, the National Health and Medical Research Council (Australia), the Medical Foundation of the University of Sydney, National Eye Institute Research grant EY00888, and the Santa Barbara Cottage Hospital. |
Vol 128 - N° 2
P. 165-172 - août 1999 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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